金莲花中荭草苷锌配合物合成、表征及其抗氧化活性研究

神经药理学报 ›› 2013, Vol. 3 ›› Issue (2): 26-34.

金莲花中荭草苷锌配合物合成、表征及其抗氧化活性研究

蒋伟, 曹欣欣, 王树林, 王书华

河北北方学院药学系，张家口，075000，中国

出版日期:2013-04-26 发布日期:2014-06-27
通讯作者: 王书华，教授，硕士生导师。主要从事中药有效成分的提取及药理研究。Email：wangshyh@126.com
作者简介:蒋伟，河北北方学院2009级药理学硕士研究生， Email: 574941084@qq.com
基金资助:
张家口市科技局资助项目（11110015D）河北北方学院重大项目(ZD1314)

Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes from Trollius Chinensis Bunge

JIANG Wei，CAO Xin-xin，WANG Shu-lin，WANG Shu-hua

College of Pharmacy, Hebei North University, Zhangjiakou, 075000, China

Online:2013-04-26 Published:2014-06-27
Contact: 王书华，教授，硕士生导师。主要从事中药有效成分的提取及药理研究。Email：wangshyh@126.com
About author:蒋伟，河北北方学院2009级药理学硕士研究生， Email: 574941084@qq.com
Supported by:
张家口市科技局资助项目（11110015D）河北北方学院重大项目(ZD1314)

摘要/Abstract

摘要： 目的：优化荭草苷锌配合物合成工艺，对荭草苷和荭草苷锌配合物进行体外抗氧化活性比较研究。方法：运用荭草苷和乙酸锌合成荭草苷锌配合物，采用元素分析法、等离子发射光谱法、紫外光谱法、红外光谱法、热重分析法、核磁共振法对荭草苷锌配合物进行结构表征。选择羟基自由基(?OH)，1，1-二苯基-2-三硝基苯肼自由基(?DPPH)产生体系为实验体系，考察荭草苷和荭草苷锌配合物的抗氧化活性。结果：荭草苷与乙酸锌配位反应形成配合物的最佳工艺为：以无水乙醇为溶剂、反应pH 8.5，反应温度65℃，搅拌时间8h。得到荭草苷锌配合物可能的化学分子式[Zn3(C21H15O11)2]?4H2O和化学结构式。荭草苷和荭草苷锌配合物都具有清除·OH，DPPH的能力，且呈一定的量效关系。在1.0~8.0μg·mL-1范围内，荭草苷锌配合物各剂量组在清除·OH，·DPPH能力上高于荭草苷配体同等剂量组。结论：荭草苷锌配合物在抗氧化活性上优于荭草苷配体。

关键词: 金莲花, 荭草苷锌配合物, 结构表征, 抗氧化

Abstract:

Objective: To optimize the synthetic process conditions of and study the antioxidation activity of orientin and orientin zinc complexes in vitro. Methods: Using elemental analyzer, plasma emission spectrometer, ultraviolet spectrometer, infrared spectrometer, thermogravimetric analyzer and nuclear magnetic resonance instrument, the structural characterization of orientin zinc complexes was observed, taking superoxide anion radicals, hydroxyl radicals, 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH)radical generation system as experimental system, to examine the antioxidant activity of orientin and orientin-zinc complexes. Results: The best coordination reaction optimum process of complexes formed by orientin and zinc acetate was shown as follows. Taking anhydrous ethanol as solvent, reaction pH was 8, reaction temperature was 65℃, and reaction time was 8 h. The orientin-zinc complexes maybe the chemical formula [Zn3(C21H15O11)2]?4H2O and chemical structural formula. Orientin and orientin-zinc complexes have clear ability of removing ·OH and ·DPPH, and the effect of orientin-zinc complexes in each dose group was more obvious than equivalent dose orientin at 1.0~8.0μg·mL-1. Conclusion: Orientin-zinc complexes is more superior than orientin on the activity of antioxidation.

Key words: trollius chinensis bunge, orientin-zinc complexes, structural characterization, antioxidation

蒋伟, 曹欣欣, 王树林, 王书华. 金莲花中荭草苷锌配合物合成、表征及其抗氧化活性研究[J]. 神经药理学报, 2013, 3(2): 26-34.

JIANG Wei, CAO Xin-xin, WANG Shu-lin, WANG Shu-hua. Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes from Trollius Chinensis Bunge[J]. ACTA NEUROPHARMACOLOGICA, 2013, 3(2): 26-34.

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