荭草苷和牡荆苷对H2O2氧化损伤人红细胞的保护作用

doi:10.3969/j.issn.2095-1396.2016.04.001

神经药理学报 ›› 2016, Vol. 6 ›› Issue (4): 1-12.DOI: 10.3969/j.issn.2095-1396.2016.04.001

• 研究论文 • 下一篇

荭草苷和牡荆苷对H2O2氧化损伤人红细胞的保护作用

王晓茹，杜云广，王书华，安芳

1. 河北北方学院，张家口，075000，中国
2. 石药集团有限公司，石家庄，050000，中国

出版日期:2016-08-26 发布日期:2016-08-31
通讯作者: 安芳，男，教授，硕士生导师；研究方向：中药有效成分的提取及药理活性研究；Tel：+86-0313-4029558，E-mail：wangshyh@126.com
作者简介:王晓茹，女，硕士研究生；研究方向：中药有效成分的提取及药理活性研究；Tel：+86-0313-4029558，Email：wangxrgf@126.com
基金资助:
河北北方学院重大项目（No.ZD1314），河北省研究生创新资助项目（No.2015-203，No.2016-288）

Protective Effects of Orientin and Vitexin on H2O2 -Induced Oxidative Stress in Human Erythrocytes

WANG Xiao-ru，DU Yun-guang，WANG Shu-hua，AN Fang

Hebei North University，Zhangjiakou，075000，China

Online:2016-08-26 Published:2016-08-31
Contact: 安芳，男，教授，硕士生导师；研究方向：中药有效成分的提取及药理活性研究；Tel：+86-0313-4029558，E-mail：wangshyh@126.com
About author:王晓茹，女，硕士研究生；研究方向：中药有效成分的提取及药理活性研究；Tel：+86-0313-4029558，Email：wangxrgf@126.com
Supported by:
河北北方学院重大项目（No.ZD1314），河北省研究生创新资助项目（No.2015-203，No.2016-288）

摘要/Abstract

摘要： 目的：探讨荭草苷和牡荆苷对人红细胞氧化损伤的保护作用及其构效关系。方法：建立过氧化氢（hydrogen peroxide，H 2 O 2 ）氧化损伤人红细胞的模型，实验随机分为正常组，H 2 O 2 氧化损伤组，荭草苷和牡荆苷（终浓度 10、20、40 μg·mL -1 ）不同剂量组，20 μg·mL -1 维生素 C（vitamin C，VC）阳性对照组。研究比较荭草苷和牡荆苷各剂量组对 1，1- 二苯基 -2- 苦肼基（1，1-diphenyl-2-picrylhydrazyl，DPPH）自由基的清除率、氧化损伤红细胞的溶血率、活性氧（reactive oxygen species，ROS）、抗氧化酶系及 ATP 酶活性、丙二醛（malonaldehyde，MDA）、高铁血红蛋白（methemoglobin，MetHb）及巯基（sulfhydryl，-SH）含量的影响，扫描电镜观察红细胞表面形态。结果：荭草苷和牡荆苷对 DPPH 自由基都具有一定的清除能力，均能有效降低红细胞溶血率，减少 ROS、MDA 及 MetHb 含量，均能不同程度保护抗氧化酶、ATP 酶活性及增加 -SH 含量（P<0.05），显著改善红细胞膜的形态，其中 10 μg·mL -1 和 20 μg·mL -1 的荭草苷的作用效果优于相同剂量的牡荆苷，而40 μg·mL -1 的荭草苷和牡荆苷对红细胞具有相同的保护作用，且与 VC 作用相当。结论：荭草苷和牡荆苷对H 2 O 2 致氧化损伤的人红细胞具有保护作用，可能是通过对抗氧自由基，抑制脂质过氧化，保护细胞内抗氧化酶活性和细胞膜蛋白上的 -SH 来实现的，且荭草苷的保护作用效果优于牡荆苷。

关键词: 荭草苷, 牡荆苷, 人红细胞, 抗氧化

Abstract: Objective：This study investigated the protective effects of orientin and vitexin on hydrogen peroxide （H 2 O 2 ）-induced oxidative stress in human erythrocytes and structure-activity relationship. Methods：Oxidative stress model induced by H 2 O 2 was established in this study. The experiment was randomly divided into normal group，H 2 O 2 group （oxidative stress group），20 μg·mL -1 of VC group as a positive control，10，20 and 40 μg·mL -1 of Orientin groups and Vitexin groups，respectively. Erythrocyte suspension was incubated with different concentrations of orientin and vitexin，and then 1，1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging ability，hemolysis rate，reactive oxygen species （ROS），antioxidant and ATP enzymes，malonaldehyde （MDA），methemoglobin （MetHb） and sulfhydryl （-SH） content were evaluated by different methods. Erythrocyte morphology was also observed with scanning electron microscope. Results：Erythrocytes pre-treated orientin or vitexin had certain DPPH-radical clearance ability，lowered hemolysis rates，reduced the content of ROS，MDA，MetHb protected antioxidative enzymes and ATPs activities and increased thiol group content in a dose-dependent manner （P<0.05）. Moreover，orientin and vitexin recovered cell surface morphogy. Comparative results showed the bio-activity of orientin was same as vitexin when the concentration was 40 μg·mL -1 for both，which was equivalent to VC at 20 μg·mL -1 . But orientin showed better biological activity than vitexin at the concentrations of 10 and 20 μg·mL -1 . Conclusion：Orientin and vitexin are able to protect human erythrocytes from oxidative stress through scavenging oxygen free radicals，inhibiting the perioxidation of lipids，protecting anti-oxidative and ATP enzymes activities and increasing-SH content. Moreover，the protective effects of orientin showed better biological activity than vitexin.

Key words: orientin, vitexin, human erythrocytes, anti-oxidative

王晓茹，杜云广，王书华，安芳. 荭草苷和牡荆苷对H2O2氧化损伤人红细胞的保护作用[J]. 神经药理学报, 2016, 6(4): 1-12.

WANG Xiao-ru，DU Yun-guang，WANG Shu-hua，AN Fang. Protective Effects of Orientin and Vitexin on H2O2 -Induced Oxidative Stress in Human Erythrocytes[J]. Acta Neuropharmacologica, 2016, 6(4): 1-12.

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荭草苷和牡荆苷对H2O2氧化损伤人红细胞的保护作用

Protective Effects of Orientin and Vitexin on H2O2 -Induced Oxidative Stress in Human Erythrocytes

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