雌二醇对去卵巢大鼠学习记忆能力及脑内氨基酸水平的影响

神经药理学报 ›› 2011, Vol. 1 ›› Issue (5): 8-13.

雌二醇对去卵巢大鼠学习记忆能力及脑内氨基酸水平的影响

张夏微，张丹参，薛贵平，赵一洁，金灿

河北北方学院药学系，张家口，07500，中国

出版日期:2011-10-26 发布日期:2013-03-24
通讯作者: 张丹参，女，教授，博士生导师；研究方向：神经药理学；Tel：+86-0313-4029556，E-mail：zhangdanshen2011@126.com
作者简介:张夏微，女，研究生；E-mail：zhangxiawei0606@163.com
基金资助:
国家自然科学基金项目（No.81274055），河北省自然科学基金项目（No.C2011405014）

Effects of estradiol on learning and memory in ovariectomized rats: relationship with the contents of amino acids in the cerebrum

ZHANG Xia-wei, ZHANG Dan-shen, XUE Gui-ping, ZHAO Yi-jie, JIN Can

Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China

Online:2011-10-26 Published:2013-03-24
Contact: 张丹参，女，教授，博士生导师；研究方向：神经药理学；Tel：+86-0313-4029556，E-mail：zhangdanshen2011@126.com
About author:张夏微，女，研究生；E-mail：zhangxiawei0606@163.com
Supported by:
国家自然科学基金项目（No.81274055），河北省自然科学基金项目（No.C2011405014）

摘要/Abstract

摘要： 目的：研究雌二醇对去卵巢大鼠学习记忆能力及脑内氨基酸水平的影响。方法：采用双侧卵巢摘除术（OVX, ovariectomy）建立大鼠雌激素缺乏模型，于OVX后第7周每日皮下注射（sc）雌二醇75, 300μg•kg-1至第16周。第15周通过Morris水迷宫检测大鼠的学习记忆能力，第16周处死大鼠，用高效液相色谱法测定大鼠前皮层和海马的4种氨基酸的含量。结果：与假手术组相比，OVX组大鼠学习记忆能力明显下降，前皮层和海马谷氨酸（Glutamic acid, Glu）含量及谷氨酸/γ-氨基丁酸（Glutamic acid/γ-aminobutyric acid, Glu/GABA）明显下降；与模型组相比，雌二醇75μg•kg-1治疗组，学习记忆能力明显改善，其前皮层和海马Glu含量及Glu/GABA明显升高；而雌二醇300μg•kg-1治疗组大鼠学习记忆能力无明显改善，其前皮层Glu/GABA明显增加，但海马Glu/GABA无明显变化。.结论：雌二醇75μg•kg-1改善去卵巢大鼠的学习记忆能力可能与其增加脑内尤其是海马Glu/GABA比值有关。

关键词: 雌二醇, 去卵巢, 高效液相色谱法, 谷氨酸, &, gamma, -氨基丁酸, 谷氨酸/&, gamma, -氨基丁酸

Abstract: Objective: To investigate the influence of estradiol on learning and memory and the content of amino acids in the cerebrum of ovariectomized rats. Methods: Adult female rats were treated daily with subcutaneous injection of estradiol (75, 300 μg•kg-1) between weeks 7 and 16 after ovariectomy (OVX) or sham operation. The learning and memory of the rats was tested by a Morris water maze task on week 15, after which they were sacrificed and the content of amino acids in the cerebrum measured with high performance liquid chromatography (HPLC). Results: The ability of learning and memory was significantly improved, and the content of glutamic acid and the ratio of glutamic acid to γ-aminobutyric acid （Glu/GABA） in prefrontal cortex and hippocampus of OVX group was significantly decreased as compared to those of sham group. In addition, the ability of learning and memory of estradiol（75 μg•kg-1）treated group was significantly improved and the content of Glu and Glu/GABA in prefrontal cortex and hippocampus were significantly increased as compared to those of OVX group. However, the Glu/GABA ratio remained unchanged in the hippocampus of estradiol (300 μg•kg-1) treated group as compared to that of OVX group; and the ability of learning and memory in these rats did not significantly improve. Conclusion: Estradiol (75 μg•kg-1) improves the ability of learning and memory in OVX rats, and the increased Glu/GABA ratio in hippocampus possibly contributes to such an effect.

Key words: estradiol, ovariectomy（OVX）, high performance liquid chromato- graphy, glutamic acid, &, gamma, -aminobutyric acid (GABA), glutamic acid/&, gamma, -aminobutyric acid (Glu/GABA)

中图分类号:

张夏微, 张丹参, 薛贵平, 赵一洁, 金灿. 雌二醇对去卵巢大鼠学习记忆能力及脑内氨基酸水平的影响[J]. 神经药理学报, 2011, 1(5): 8-13.

ZHANG Xia-wei, ZHANG Dan-shen, XUE Gui-ping, ZHAO Yi-jie, JIN Can. Effects of estradiol on learning and memory in ovariectomized rats: relationship with the contents of amino acids in the cerebrum[J]. ACTA NEUROPHARMACOLOGICA, 2011, 1(5): 8-13.

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