实验性阿尔茨海默病小鼠海马中磷酸酶及张力蛋白同源基因水平的变化

神经药理学报 ›› 2011, Vol. 1 ›› Issue (2): 19-23.

实验性阿尔茨海默病小鼠海马中磷酸酶及张力蛋白同源基因水平的变化

徐剑文^1,2，杨渐¹，俞昌喜¹

1. 福建医科大学药学院药理学系，福州，350004，中国 2. 福建省南平市第二医院，南平，354200，中国

出版日期:2011-04-26 发布日期:2012-04-20
通讯作者: 俞昌喜，男，教授，博士生导师；研究方向：神经精神药理学；Tel：+86-0591-83569311,?E-mail：changxiyu@mail.fjmu.edu.cn
作者简介:徐剑文，男，硕士，主治医师；研究方向：阿尔茨海默病研究
基金资助:
福建省自然科学基金重点项目（No.C0820002）

Changes of PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome 10) Gene Level in the Hippocampus of Experimental Alzheimer’s Disease Mice

XU Jian-wen^1，2, YANG Jian¹, YU Chang-xi¹

1. Department of Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, 350004, China 2. Second Hospital of Nanping City, Nanping, 354200, China

Online:2011-04-26 Published:2012-04-20

摘要/Abstract

摘要： 目的 : 探讨铝毒性拟阿尔茨海默病（Alzheimer’s disease, AD）小鼠诱导发生发展过程中小鼠海马组织中磷酸酶与张力蛋白同源基因（phosphatase and tensin homolog deleted on chromosome 10，PTEN）以及磷酸化PTEN 水平的动态变化。方法：侧脑室注射（icv）0.5% AlCl₃ 溶液（溶解在人工脑脊液中）2 μL，每天 1 次，连续 5 d，诱导实验性 AD 小鼠模型；末次 icv 后 d 2、d 7 采用避暗法，d 23~d 30 天采用 Morris 水迷宫法检测小鼠学习记忆能力；Western Blotting 法测定小鼠海马组织 PTEN 及磷酸化 PTEN 蛋白的水平。结果 : 模型组小鼠被动回避能力及空间学习记忆能力呈进行性障碍。在末次 icv 后 d 2、d 7 2 个时间点上，模型组小鼠脑内海马组织 PTEN、磷酸化 PTEN-Ser³⁸⁰ 蛋白水平与对照组比较均未见显著差异；而在 d 30 时间点上，模型组小鼠海马组织 Ser³⁸⁰ 位点磷酸化 PTEN 蛋白水平显著低于对照组，但 PTEN 蛋白水平未有显著差异。结论 : 铝毒性拟 AD 小鼠模型发展后期其海马组织磷酸化 PTEN 蛋白水平显著降低。

关键词: 阿尔茨海默病, 磷酸酶及张力蛋白同源基因, 磷酸化 PTEN, 海马, 小鼠, 学习记忆

Abstract: Objective: To investigate the dynamic alteration of PTEN（Phosphatase and Tensin Homolog Deleted on Chromosome 10）and phospho-PTEN levels at different stages of mimic Alzheimer’s disease（AD）mice induced by AlCl3. Methods：AD-like mouse was made by intracere-broventricular injection（icv）of 2 μL of 0.5% AlCl₃ dissolved in artificial cerebrospinal fluid, once a day, consecutively for 5 d. Passive avoidance response test was used at d 2 and d 7, and Morris water maze test at d 23 to d 30 after the last icv injection to detect the learning and memory capabilities of mice. Western Blotting was used to detect the protein levels of PTEN and phospho-PTEN in the hippocampus of mice. Results: The results of step through passive avoidance response showed a significant retardation of the model mice from d 2 to d 7 after the last icv injection compared with that of the control group; from d 23 to d 30 after the last icv injection, Morris water maze（MWM） performance displayed a significant impairment in learning and memory ability of model mice. No significant differences on protein levels of PTEN and phospho-PTEN in the hippocampus were found between the model mice and control mice at the d 2 and d 7 time-points after the last icv injection. At the d 30 time-point after the last icv injection, the protein level of phospho-PTEN-Ser³⁸⁰ of the model mice was significantly lower than that of the control group, but no significant difference about PTEN protein level. Conclusion：The phospho-PTEN protein level of hippocampus in the late development stage of mimic AD mice induced by aluminum toxicity is decreased significantly.

Key words: Alzheimer&, rsquo, s disease, PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome 10) phospho-PTEN, hippocampus, mice, learning and memory

中图分类号:

R749.16

徐剑文，杨渐，俞昌喜. 实验性阿尔茨海默病小鼠海马中磷酸酶及张力蛋白同源基因水平的变化[J]. 神经药理学报, 2011, 1(2): 19-23.

XU Jian-wen, YANG Jian, YU Chang-xi. Changes of PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome 10) Gene Level in the Hippocampus of Experimental Alzheimer’s Disease Mice[J]. Acta Neuropharmacologica, 2011, 1(2): 19-23.

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