高压静电法制备微胶囊化人视网膜色素上皮细胞

神经药理学报 ›› 2011, Vol. 1 ›› Issue (2): 24-27.

高压静电法制备微胶囊化人视网膜色素上皮细胞

高冬晓，赵亚群

中国人民解放军第三0九医院神经外科，北京，100091，中国

出版日期:2011-04-26 发布日期:2012-04-20
通讯作者: 赵亚群，男，硕士，副主任医师，硕士生导师；研究方向：立体定向神经外科；Tel：+86-010-66775066，E-mail：zhaoyaqun2@sina.com
作者简介:高冬晓，女，硕士；研究方向：神经药理学；Tel：+86-010-66775066，E-mail:gaodongxiao08@yeah.net
基金资助:
北京市海淀区科委项目（No.0831）

Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method

GAO Dong-xiao，ZHAO Ya-qun

Department of Neurosurgery, 309^th Hospital of PLA, Beijing, 100091, China

Online:2011-04-26 Published:2012-04-20

摘要/Abstract

摘要： 目的：探索用高压静电法制备人视网膜色素上皮细胞（human retinal pigment epithelial cell，hRPE）海藻酸钠-壳聚糖-海藻酸钠（Alginate-Chitosan-Alginate，ACA）微胶囊。方法：用自制的高压静电微胶囊发生装置制备包被hRPE细胞的ACA微胶囊，并观察所得细胞微胶囊在培养液中培养1周的细胞总数及存活率。结果：制备出直径为（400±13）μm的hRPE细胞ACA微胶囊。d 1~d 3微胶囊内细胞总数及存活率变化不显著，而培养到d 7后总数显著上升，存活率降低，但总的活细胞数增加。结论：用自制的高压静电微胶囊发生装置能成功制备出hRPE细胞的ACA微胶囊；包被在微胶囊中的细胞培养1周后，细胞增殖显著。

关键词: 高压静电法, 微胶囊化, 人视网膜色素上皮细胞

Abstract: Objective: To prepare alginate-chitosan-alginate（Alginate-Chitosan-Alginate, ACA） microencapsulated Human Retinal Pigment Epithelial（hRPE） cells using high voltage electrostatic method. Methods: The microencapsulated hRPE was prepared by using our home-made high voltage electrostatic system, and the total number and the survival rate of microencapsulated hRPE cells were studied in the culture media for 7 d. Results: hRPE cells ACA microcapsule with diameter of （400±13） μm were obtained. the total number and the survival rate of cells had no statistically significant differences at the first 3 d. But after 7 d the total number of cells and the survival number were increased and the survival rate of cells was reduced. Conclusion: hRPE cells ACA microcapsule can be obtained with high voltage electrostatic method; after 7 d, the microencapsulated cells grow faster.

Key words: high voltage electrostatic preparation method, microencapsulated, human reti-nal pigment epithelial Cell（hRPE）

中图分类号:

高冬晓，赵亚群. 高压静电法制备微胶囊化人视网膜色素上皮细胞[J]. 神经药理学报, 2011, 1(2): 24-27.

GAO Dong-xiao，ZHAO Ya-qun. Production of Microencapsulated Human Retinal Pigment Epithelial Cells by the High Voltage Electrostatic Method[J]. Acta Neuropharmacologica, 2011, 1(2): 24-27.

参考文献

1 Olle Lindvall, Anders Björklund. Cell therapeutics in Parkinson’s disease[J]. Neurotherapeutics, 2011, 8(4): 539-548.
2 Dunnett Stephen B, Rosser Anne E. Clinical translation of cell transplantation in the brain[J].Curr Opin Organ Transplant,2011, 16(6): 632-639.
3 Thaddevs P.Dryja, Maureen O’Neil-Dryia, John M.Pawelek, et al. Demonstration of tyrosinase in the adult bovine uveal tract and retinal pigment epithelium[J]. Cell Transplantation, 1978, 17:511-514.
4 Smith SB, Zhou BK, Orlow SJ. Expression of tyrosinase and the tyrosinase related Proteins in the Mitfvit(vitiligo) mouse eye: implications for the function of the microphthalmia transcription factor[J]. Exp. Eye Res, 1998, 66:403-410.
5 李锐, 宗伟, 郭民侠, 等. 微囊化人视网膜色素上皮细胞移植治疗帕金森病的实验研究[J]. 中华神经医学杂志, 2009, 8(2): 125-128.
6 杜望春, 刘学源, 陈玉娟, 等. 微囊化牛视网膜色素上皮细胞体外BDNF及GDNF分泌特性的研究[J]. 同济大学学报(医学版）,2008, 29(4): 22-26.
7 Sun Mei, Kong Linxin, Wang Xiaodan, et al.Comparison of the capability of GDNF, BDNF, or both, to protect nigrostriatal neurons in a ratmodel of Parkinson’s disease[J]. Brain Reseach, 2005, 1052 (2):119 – 129.
8 Thyagarajan Subramanian. Cell Transplantation for the Treatment of Parkinson's Disease[J]. Seminars in Neurology, 2001, 21(1): 103-115.
9 Shafer TJ , Atchison WD. Transmitterion channel and receptor properties of pheochromocytoma (PC12) cells: a model for neuro toxicological studies[J]. Neurotoxicology, 1991, 12(3): 473-492.
10 Brolongan CV, Cameron DF. CNS immunological modulation of neural graft rejection and survival[J]. Neuro Res, 1996, 18: 297-301.
11 李卫, 徐如祥, 陈善成, 等. 大鼠脑组织损伤后局部及血液中细胞免疫状况的改变[J]. 中华神经医学杂志, 2006，5(6):566-568.
12 Zhang Houliang, Wu Jianjun, Ren Huimin. Therapeutic effect of microencapsulated porcine retinal pigmented epithelial cells transplantation on rat model of Parkinson’s disease[J]. Neurosc Bull , 2007, 23(3): 137-144.