Objective：Thin layer separation and qualitative identification of alcohol extract of Taohong Siwu decoction. Methods：Thin layer chromatography （TLC） was used to observe the development of chemical components in the alcohol extract of Taohong Siwu decoction，and iodine vapor was used to perform color rendering on the spots，and the thin layer plate was placed in a thin-layer scanner for scanning. The absorption spectra and retardation factor （Rf）values of the spots were recorded and compared with the reference substances. The silica gel G thin laminate was used as the stationary phase，and n-butanol-acetic acid-water （4∶1.5∶5，upper layer） was used as the developing agent，and examined under UV lamp （365 nm）. Results：A total of 5 spots were isolated from the alcohol extract of Taohong Siwu decoction，among which 3 spots were qualitatively identified as hydroxyl safflower yellow A （from Carthami Flos），paeoniflorin（from Paeoniae Radix Alba），and ferulic acid （from Angelicae Sinensis Radix and ChuanXiong Rhizoma）. The other 2 spots were not detected in the six single drugs of Taohong Siwu decoction. Conclusion：TLC can be used to separate the chemical constituents from the alcohol extract of Taohong Siwu decoction，and on this basis，new constituents can be further identified. The separation method is simple，rapid，stable，and feasible.