The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets

Acta Neuropharmacologica ›› 2012, Vol. 2 ›› Issue (6): 1-17.

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The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets

ZHANG Dan-Shen

Hebei University of Science and Technology, Shijiazhuang, 050018, China

Online:2012-12-26 Published:2014-06-27
About author:张丹参,女,教授,博士,博士生导师；研究方向:中药有效成分抗衰老作用。Tel/Fax:86-311-81668016,E-mail:zhangds2011@126.com
Supported by:
国家自然科学基金项目（No. 81274005、No.81073120）,河北省科技攻关计划项目（No.C2011405014）

Abstract

Abstract: Objective: On the basis of the previous studies, and further evaluate and research the bioactive components group of Radix Salviae Miltiorrhiae (RXM) and compound Danshen tablets by multifactor and multilevel Uniform design-High throughput screening method,to find a new pattern to research traditional Chinese medicine. Methods:By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector,11 of bioactive components which been confirmed with pharmacological activities of RXM and compound Danshen tablets(4 of water-solubility and 7 of liposolubility) and Compound Danshen Tablets (6 were recombined to be a new compatible group through uniform design. And the effects of the anti-oxidant microplate quantification experiment modles were detected by anti-DPPH effect,eliminating ratio of hydroxy free radical,reduction ability determination,and lipid peroxidation were established. Results:After high-throughout screening and rescreening, better water-solubility, better water-solubility add liposolubility,and optimal combination compatibility of RXM were obtained. Conclusion:The results indicated that effective component of RXM compatibility of different concentrations of uniform design,and with Uniform design-high throughput screening of anti-oxidant models of microplate quantification was referred to research bioactive component of compound recipe of traditional Chinese medicine.

Key words: Uniform design-High throughput screening(UD-HTS), Radix Salviae Miltiorrhiae(Danshen,RXM), Water soluble constituents, Fat-soluble components, proportion

ZHANG Dan-Shen. The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets[J]. Acta Neuropharmacologica, 2012, 2(6): 1-17.

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The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets

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