Abstract: Objective: To investigate the effects of chlorogenic acid on granular cell in cerebellum induced by amyloid β- protein -induced macrophage activation in vitro. Methods: The experiment was divided into blank control group, Aβ_25-35 treatment group and Aβ_25-35+CHA co-processing of different time group; Blank control group: Equal amount of culture medium; Aβ_25-35 treatment group: with Aβ_25-3510 μmol·L^-1; Aβ_25-35 and different time of CHA treatment group; The co-cultured system of cerebellar granular cells and macrophages were cultivated for 2 days and then the 10 μmol·L^-1 Aβ_25-35 and 1000 μmol·L^-1 CHA were added into this system for 6, 12, 24, 48, 96 h respectively. The growth phynotype of neurons and macrophages were detected by inverted phase contrast microscope; The expressions of p38MAPK, MAPKAPK-2, HSP27 protein were tested by Western blot. Results: The numbers cerebellar granule cell of chlorogenic acid protection group was increased significantly compared with Aβ_25-35 injury group. CHA could inhibite increased expression of phosphorylated p38MAPK of macrophage induced by Aβ_25-35 at difference time; Decreased expression of phosphorylated MAPKAPK-2 and phosphorylated HSP-27of macrophage induced by Aβ_25-35 could increased after the 1000 μmol·L^-1 CHA added into the medium. Conclusion: The protection of Chlorogenic acid for cerebellar granular cells induced by Aβ_25-35 via inhibition the activation of p38MAPK signaling pathway, which decreased the expression of p38MAPK and increased the expression of MAPKAPK-2, HSP-27.

Key words: chlorogenic acid, A&beta, _25-35, CGC, macrophage, p38MAPK, MAPKAPK-2, HSP27