神经药理学报

神经药理学报 ›› 2021, Vol. 11 ›› Issue (4): 4-.DOI: 10.3969/j.issn.2095-1396.2021.04.002

• 研究论文 • 上一篇    下一篇

探究沙棘油对人胃癌细胞HGC-27增殖与凋亡的影响

白鲁根，贺小霞   

  1. 1. 靖边县人民医院检验科，靖边，718500，中国

    2. 靖边县中医医院，靖边，718500，中国

  • 出版日期:2021-08-26 发布日期:2021-08-26
  • 通讯作者: 贺小霞，主管检验技师；E-mail：15686735132@qq.com
  • 作者简介:白鲁根，硕士研究生，主管检验技师；研究方向：医学基础研究；E-mail：yysw1122@qq.com
  • 基金资助:

    2019 年榆林市科协青年人才托举计划项目（No.20190111）

The Effect of Sea Buckthorn Oil on Cell Proliferation and Apoptosis of Human Gastric Cancer Cells HGC-27

BAI Lu-gen 1，HE Xiao-xia 2   

  1. 1. Department of Laboratory Medicine，Jingbian County People’s Hospital，Jingbian，718500，China

    2. Jingbian County Hospital of Traditional Chinese Medicine，Jingbian，718500，China

  • Online:2021-08-26 Published:2021-08-26

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摘要：

目的：探究沙棘油对人胃癌HGC-27 细胞增殖与凋亡的影响。方法：使用沙棘油干预人胃癌细胞HGC-27 细胞，使用CCK-8 试剂盒检测细胞活性、Western Blotting 实验检测凋亡基因P53、P21、Bcl-2 蛋白表达水平。结果：细胞活性实验显示，小于等于0.1% 助溶剂二甲基亚砜（dimethyl sulfoxide，DMSO）细胞培养液对胃癌HGC-2 细胞活性无影响，使用DMSO 溶解沙棘油干预胃癌细胞，当沙棘油浓度为5、10、20、40 μg·mL-1 均能够较好的抑制HGC-27 细胞的增殖（P<0.05）。Western Blotting 结果显示，较对照组当沙棘油浓度为10、20、40 μg·mL-1 时，P53 蛋白表达量均明显增加；较对照组当沙棘油浓度为5、10、20、40 μg·mL-1 时，Bcl-2 蛋白表达量均明显降低，P21 蛋白表达量均明显升高（P<0.05）。结论：沙棘油可通过P53 信号通路抑制人胃癌细胞增殖，促进胃癌细胞凋亡，达到抗肿瘤作用。

关键词: HGC-27, P53；沙棘油；P21

Abstract:

Objective：To explore the effect of sea buckthorn oil on cell proliferation and apoptosis of human gastric cancer cells HGC-27. Methods：HGC-27 cells were treated with sea buckthorn oil. The cell viability was measured by CCK-8 kit. Western blotting was used to detect the protein expression of p53，p21 and Bcl-2. Results：Cell viability tests results showed that there is no effect on HGC-27 cell viability when DMSO content in cell culture medium is less than or equal to 0.1%. HGC-27 cells were treated with sea buckthorn oil dissolved in DMSO. The proliferation of HGC-27 cells was inhibited by sea buckthorn oil at the concentration of 5，10，20，

40 mol·L-1（P<0.05）. Compared with control group，the expression level of p53 in three different concentration of sea buckthorn oil （10，20，40 mol·L-1） group were significantly increased（P<0.05）. The expression level of Bcl-2 was significantly decreased and p21 was significantly increased in the concentration of sea buckthorn oil （5，10，20，40 mol·L-1） group. Conclusion：Sea buckthorn oil can inhibit HGC-27 proliferation and promote apoptosis by p53 signaling pathway，and has anti-cancer effect.

Key words: HGC-27, P53, sea buckthorn oil, P21

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