再灌注早期高血糖加重脑卒中脑糖的增加

神经药理学报 ›› 2015, Vol. 5 ›› Issue (4): 1-7.

• 研究论文 • 下一篇

再灌注早期高血糖加重脑卒中脑糖的增加

张帅，夏聪媛，周恒，杨鹏飞，陈乃宏

中国医学科学院药物研究所&神经科学中心，天然药物活性物质与功能国家重点实验室，北京，100050，中国

出版日期:2015-08-26 发布日期:2016-03-03
通讯作者: 陈乃宏，男，研究员，博士生导师；研究方向：神经精神药理学；Tex：+86-010-63165182，Fax：+86-010-63165177，E-mail：chennh@imm.ac.cn
作者简介:张帅，男，博士研究生；研究方向：神经精神药理学；E-mail：zhangshuai2586@163.com
基金资助:
国家自然科学基金项目（No.81274122、81173578、81202507、81373997），国家“重大新药创制”科技重大专项项目（2012ZX09301002-004、2012ZX09103101-006、2012ZX09301002-001），国家高技术研究发展计划（863 计划）项目（2012AA020303），教育部长江学者和创新团队发展计划（PCSIRT）项目（IRT1007），教育部博士点基金重点项目（No. 20121106130001），北京市自然科学基金（No.7131013、7142115），新药作用机制研究与药效评价北京市重点实验室资助项目（No.BZ0150）

Hyperglycemia in Acute Reperfusion Aggravates Cerebral Glucose Increase after Stroke

ZHANG Shuai, XIA Cong-Yuan, ZHOU Heng, YANG Peng-Fei, CHEN Nai-Hong

State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences, Beijing, 100050, China

Online:2015-08-26 Published:2016-03-03
Contact: 陈乃宏，男，研究员，博士生导师；研究方向：神经精神药理学；Tex：+86-010-63165182，Fax：+86-010-63165177，E-mail：chennh@imm.ac.cn
About author:张帅，男，博士研究生；研究方向：神经精神药理学；E-mail：zhangshuai2586@163.com
Supported by:
国家自然科学基金项目（No.81274122、81173578、81202507、81373997），国家“重大新药创制”科技重大专项项目（2012ZX09301002-004、2012ZX09103101-006、2012ZX09301002-001），国家高技术研究发展计划（863 计划）项目（2012AA020303），教育部长江学者和创新团队发展计划（PCSIRT）项目（IRT1007），教育部博士点基金重点项目（No. 20121106130001），北京市自然科学基金（No.7131013、7142115），新药作用机制研究与药效评价北京市重点实验室资助项目（No.BZ0150）

摘要/Abstract

摘要： 目的：观察在缺血性脑卒中发生后，高血糖对脑糖水平及脑卒中损伤的影响。方法：采用线栓栓塞大脑中动脉诱发大脑局灶性脑缺血模型模拟缺血性脑卒中，在再灌注前5 min单次腹腔注射50%葡萄糖溶液（6 mL•kg-1）诱导再灌注早期高血糖。在再灌注30 min和24 h时检测脑糖水平，并通过神经功能评分及脑梗死面积的统计评价脑缺血再灌注损伤。结果：给予葡萄糖后，血糖显著增加，并持续至再灌注后120 min （P < 0.05）。缺血再灌注损伤可引起脑糖水平的增加，再灌注早期血糖的升高会进一步加重再灌注24 h时脑糖水平的增加，并显著增加再灌注24 h时神经功能评分和脑梗死面积（P < 0.05）。结论：脑缺血后，再灌注早期高血糖可加重再灌注后脑糖水平和脑损伤的增加，脑糖水平的升高可能是高血糖介导的脑卒中损伤加重的直接原因。

关键词: 脑卒中, 缺血再灌注, 高血糖, 脑糖

Abstract: Objective: To explore the effects of hyperglycemia on cerebral glucose level and brain injury after ischemic stroke. Methods: The middle cerebral artery occlusion (MCAO) model was used to mimic the ischemic stroke. Hyperglycemia in acute reperfusion was induced by single intraperitoneal injection of 50% glucose (6 mL•kg-1) at 5 min before reperfusion in MCAO-treated rats. After reperfusion for 30 min and 24 h, the cerebral glucose levels were detected, and the neurological score and infarct areas were assessed. Results: After glucose administration, the blood glucose increased significantly and lasted for 120 min after reperfusion (P<0.05). Ischemia and reperfusion injury caused cerebral glucose increase. Hyperglycemia in acute reperfusion significantly aggravated the elevation of cerebral glucose, and increased the neurological score and infarct areas after reperfusion for 24 h (P<0.05). Conclusion: After cerebral ischemia, hyperglycemia in acute reperfusion could aggravate cerebral glucose increase and stroke injury. The elevation of cerebral glucose levels might directly mediate aggravation of stroke injury caused by hyperglycemia.

Key words: stroke, ischemia and reperfusion, hyperglycemia, cerebral glucose

张帅，夏聪媛，周恒，杨鹏飞，陈乃宏. 再灌注早期高血糖加重脑卒中脑糖的增加[J]. 神经药理学报, 2015, 5(4): 1-7.

ZHANG Shuai, XIA Cong-Yuan, ZHOU Heng, YANG Peng-Fei, CHEN Nai-Hong. Hyperglycemia in Acute Reperfusion Aggravates Cerebral Glucose Increase after Stroke[J]. Acta Neuropharmacologica, 2015, 5(4): 1-7.

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再灌注早期高血糖加重脑卒中脑糖的增加

Hyperglycemia in Acute Reperfusion Aggravates Cerebral Glucose Increase after Stroke

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