神经药理学报

神经药理学报 ›› 2016, Vol. 6 ›› Issue (3): 17-23.DOI: 10.3969/j.issn.2095-1396.2016.03.003

• 实验方法学 • 上一篇    下一篇

噁唑酮与三硝基苯磺酸联用诱导小鼠慢性溃疡性结肠炎模型

宋晓敏，张丹参，庄忠宝，张海威，苏晓梅，赵凯燕，张力   

  1. 1.河北北方学院药学系，张家口，075000，中国
    2.河北科技大学，石家庄，050000，中国
  • 出版日期:2016-06-26 发布日期:2016-05-10
  • 通讯作者: 张力，男，研究生导师；研究方向：神经药理学；E-mail：hbnulzhang@126.com
  • 作者简介:宋晓敏，女，硕士研究生；研究方向：临床药理学；E-mail：songxm2016@126.com 张丹参，女，博士生导师；研究方向：神经药理学；E-mail：zhangds2011@126.com
  • 基金资助:

    国家自然基金课题（No.81274005），河北省自然科学基金项目（No.H2016208058），河北省教育厅科学技术研究重点项目（No.
    ZD2016009）

Chronic Ulcerative Colitis Model Induced by Oxazolone and 2,4,6-Trinitrobenzene Sulfonic Acid in Mice

SONG Xiao-min, ZHUANG Zhong-bao, ZHANG Hai-wei, SU Xiao-mei, ZHAO Kai-yan, ZHANG Li   

  1. 1. Department of pharmacology，Hebei North University, Zhangjiakou, 075000, China 2.Hebei University of Science and Technology, Shijiazhuang, 050000, China  
  • Online:2016-06-26 Published:2016-05-10
  • Contact: 张力，男，研究生导师；研究方向：神经药理学；E-mail：hbnulzhang@126.com
  • About author:宋晓敏，女，硕士研究生；研究方向：临床药理学；E-mail：songxm2016@126.com 张丹参，女，博士生导师；研究方向：神经药理学；E-mail：zhangds2011@126.com
  • Supported by:

    国家自然基金课题（No.81274005），河北省自然科学基金项目（No.H2016208058），河北省教育厅科学技术研究重点项目（No.
    ZD2016009）

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摘要： 目的：采用噁唑酮（oxazolone, OXZ）与2，4，6-三硝基苯磺酸（2, 4, 6-trinitrobenzene sulfonic acid, TNBS）结合诱导小鼠慢性溃疡性结肠炎模型。方法：取24只健康成年雄性昆明（KM）小鼠，随机分为两组，正常对照组（N组，n=12），模型组（M组，n=12），按照一定的周期诱导方法对小鼠进行OXZ与TNBS灌肠，并每天记录小鼠精神状态、毛色、进食、大便情况并检测小鼠疾病活动指数（disease activity index，DAI）值。造模周期结束之后，N组与M组每周每组处死三只小鼠，分别记为N1、N2、N3、N4、M1、M2、M3和M4组。分离结肠，测定小鼠结肠组织大体评分（colon macroscopic damage index, CMDI）值以及结肠湿重指数（colon index, CI），采用免疫组化技术检测病变部位白细胞介素-4（interleukin-4, IL-4）和肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）的水平。结果：正常组小鼠各项评分稳定，M1、M2、M3和M4模型组小鼠各项评分均显著高于正常组。结论：噁唑酮与三硝基苯磺酸联用可成功诱导出更接近人类慢性溃疡性结肠炎的小鼠模型，病变可维持一月。

关键词: 溃疡性结肠炎, 慢性, 噁唑酮, 2, 4, 6- 三硝基苯磺酸

Abstract: Objective: To establish the chronic ulcerative colitis model induced by oxazolone and 2,4,6-trinitrobenzene sulfonic acid together in mice. Methods: 24 healthy male Kunming (KM) mice were randomly divided into two groups: normal control group (N group, n=12), model group (group M, n=12). The mice were treated with OXZ and TNBS according to a certain cycle induction method by rectally administration, and the mental state, and the condition of the fur color, eating and defecation were recorded every day, and the disease activity index (disease activity index, DAI) was detected every day as well. Three mice were killed in N group and M group respectively every week, which was recorded N1, N2, N3, N4, M1, M2, M3 and M4 group after the modeling cycle. Meanwhile the colon was separated out, and the CMDI and the CI was determined. The level of the IL-4 and TNF-α detected in Immunohistochemical technique was confirmed. Results: The scores of normal mice were stable, and the scores of M1, M2, M3 and M4 model group were significantly higher than those of normal group. Conclusion: The chronic ulcerative colitis model induced by oxazolone and 2,4,6-trinitrobenzene sulfonic acid together in mice was established successfully. Its pathological characteristics are closer to the human chronic ulcerative colitis and can be kept for a month at least.

Key words: ulcerative colitis, chronic, oxazolone, 2,4,6- three trinitrobenzene sulfonic acid