Objective: To establish liquid chromatography methods for three index components of Qidan Lixin Pills and thin-layer chromatography methods for three medicinal materials. Methods: The thin-layer chromatography (TLC) method was adopted to qualitatively identify Codonopsis pilosula and Acanthopanax senticosus in Qidan Lixin Pills. The contents of salvianolic acid B, naringin and neohesperidin in Qidan Lixin Pills were determined by high performance liquid chromatography. Chromatographic column: The chromatographic column was Waters XTerra MS C18 5 μm 4.6×250 mm, the mobile phase was acetonitrile -0.1% phosphoric acid aqueous solution (22:78), the detection wavelength was 283 nm, the flow rate was 1.0mL·min^-1, and the column temperature was 30 ℃ . The theoretical plate count, calculated based on the peak of salvianolic acid B, should be no less than 6 000, and calculated based on the peaks of naringin and neohesperidin, should be no less than 3 000. Results: The qualitative identification of Codonopsis pilosula and Acanthopanax senticosus exhibited clear and distinct spots with no interfering signals observed. In the quantitative analysis, salvianolic acid B, naringin, and neohesperidin demonstrated good linearity within the injection ranges of 0.300 3~3.003 1 μg, 0.135 8~1.358 2 μg, and 0.125 6~1.255 5 μg, respectively. The average recoveries were 102.53%, 99.37%, and 100.48%, with corresponding relative standard deviations RSD of 2.20%, 1.23%, and 1.08% (n = 6), indicating satisfactory precision and accuracy. Conclusion: The method established in this experiment has good repeatability, is stable and feasible, and can be used for the quality control of Qidan Lixin Pills.