2’- 溴-2- 羟基-4，6，4’，5’- 四甲氧基查尔酮抑制SK-N-SH 细胞增殖的作用研究

doi:10.3969/j.issn.2095-1396.2016.01.003

神经药理学报 ›› 2016, Vol. 6 ›› Issue (1): 18-24.DOI: 10.3969/j.issn.2095-1396.2016.01.003

2’- 溴-2- 羟基-4，6，4’，5’- 四甲氧基查尔酮抑制SK-N-SH 细胞增殖的作用研究

刘军举，付冬君，牛进波，张旗，张雁冰

郑州大学药物研究院，郑州，450001，中国

出版日期:2016-02-26 发布日期:2016-05-10
通讯作者: 张雁冰，女，博士，教授，博士生导师；研究方向：有机化学及天然药物化学；E-mail：zhangyb@zzu.edu.cn
作者简介:刘军举，男，硕士研究生；研究方向：神经药理及分子肿瘤药理；E-mail：114074252@qq.com
基金资助:
国家自然科学基金资助项目（No.81273393）

Antiproliferation Effects of 2’-bro-2-hydroxy-4,6,4’,5’-tetramethoxychalcone on Human Neuroblastoma SK-N-SH Cells

LIU Jun-ju，FU Dong-jun，NIU Jin-bo，ZHANG Qi，ZHANG Yan-bing

School of Pharmaceutical Sciences，Zhengzhou University，Zhengzhou，450001，China

Online:2016-02-26 Published:2016-05-10
Contact: 张雁冰，女，博士，教授，博士生导师；研究方向：有机化学及天然药物化学；E-mail：zhangyb@zzu.edu.cn
About author:刘军举，男，硕士研究生；研究方向：神经药理及分子肿瘤药理；E-mail：114074252@qq.com
Supported by:
国家自然科学基金资助项目（No.81273393）

摘要/Abstract

摘要： 目的：研究 2’- 溴 -2- 羟基 -4，6，4’，5’- 四甲氧基查尔酮（ 2’-bro-2-hydroxy-4，6，4’，5’-tetramethoxychalcone，C30）的抗人神经母细胞瘤细胞（SK-N-SH）作用及其机制，为查尔酮类新型化合物开发用于神经母细胞瘤的预防和治疗提供初步的实验依据。方法：磺酰罗丹明B（sulforhodamine B，SRB）法检测药物作用肿瘤细胞后的细胞活性；流式细胞术检测细胞周期和凋亡；Hoechst 33258 染色法检测细胞凋亡。结果：C30 在1.5625~25.0000 μmol·L-1 的浓度范围内对SK-N-SH 细胞有较强的抑制作用，抑制效应在此范围内呈剂量和时间依赖性。且C30 使SK-N-SH 细胞的周期阻滞于G1 期，并可以诱导SK-N-SH 细胞发生凋亡。结论：查尔酮类新型化合物C30 能抑制SK-N-SH细胞增殖，作用机制可能与其改变SK-N-SH细胞周期分布和诱导细胞凋亡相关。

关键词: 人神经母细胞瘤, 查尔酮, 细胞增殖, 细胞周期, 凋亡

Abstract: Objective：We studied the anti-proliferation effects of 2’-bro-2-hydroxy-4，6，4’，5’-tetrametho-xychalcone（C30） on human neuroblastoma and its mechanism to provide preliminary experimental evidence for its potential application to the prevention and treatment of neuroblastoma. Methods：The antiproliferation effects of the compounds on tumor cells were detected by sulforhodamine B（SRB） assay. Cell cycle distributions were measured by flow cytometry. Cellular apoptosis was detected by Hoechst 33258 staining assay and flow cytometry. Results：C30 restrained the proliferative effect of SK-N-SH cells in a dose- and time- dependent manner. After treated with C30，the cell cycle of SK-N-SH was arrested in G1 phase. Hoechst 33258 staining and flow cytometry analysis revealed that petasin could induce apoptosis in SKN-SH cells. Conclusion：C30 can inhibit the proliferation of SK-N-SH，and the mechanism maybe related to changing cell cycle distribution and inducing apoptosis.

Key words: neuroblastoma, chalcone, cell proliferation, cell cycle, apoptosis

中图分类号:

R965

刘军举，付冬君，牛进波，张旗，张雁冰. 2’- 溴-2- 羟基-4，6，4’，5’- 四甲氧基查尔酮抑制SK-N-SH 细胞增殖的作用研究[J]. 神经药理学报, 2016, 6(1): 18-24.

LIU Jun-ju，FU Dong-jun，NIU Jin-bo，ZHANG Qi，ZHANG Yan-bing. Antiproliferation Effects of 2’-bro-2-hydroxy-4,6,4’,5’-tetramethoxychalcone on Human Neuroblastoma SK-N-SH Cells[J]. Acta Neuropharmacologica, 2016, 6(1): 18-24.

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2’- 溴-2- 羟基-4，6，4’，5’- 四甲氧基查尔酮抑制SK-N-SH 细胞增殖的作用研究

Antiproliferation Effects of 2’-bro-2-hydroxy-4,6,4’,5’-tetramethoxychalcone on Human Neuroblastoma SK-N-SH Cells

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