TreadScan步态分析系统在评价大鼠脊髓挫伤模型中的应用

神经药理学报 ›› 2012, Vol. 2 ›› Issue (5): 8-18.

TreadScan步态分析系统在评价大鼠脊髓挫伤模型中的应用

张思¹ 顾兵 ¹ 王烁宇 ² 李华南² 张国福² 张水印¹

1. 江西科技师范大学生命科学学院,南昌,330013,中国
2. 江西中医药大学附属医院脊柱外科,南昌,330006,中国

出版日期:2012-10-26 发布日期:2014-06-27
通讯作者: 顾兵，男，博士后，教授，硕士生导师；研究方向：神经精神药物学和运动人体科学；E-mail: bguemory@hotmail.com
作者简介:肖寒贫，女，体育教育训练学硕士研究生
基金资助:
国家自然科学基金资助项目（No.30960448），江西省自然科学基金项目（No.20114BAB205033），江西省教育厅科技项目（No.GJJ11596、GJJ12584）

Assessment of TreadScan Gait Analysis System in the Evaluation of Rat Spinal Cord Contusion Model

Zhang Si, GU Bing1 WANG Shuo-yu2, LI Hua-nan2, ZHANG Guo-fu, ZHANG Shui-yin1,

1. College of Life Science, Jiangxi Science & Technology Normal University, Nanchang, 330013, China 2. Department of Spine Surgery, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, China

Online:2012-10-26 Published:2014-06-27
Contact: 顾兵，男，博士后，教授，硕士生导师；研究方向：神经精神药物学和运动人体科学；E-mail: bguemory@hotmail.com
About author:肖寒贫，女，体育教育训练学硕士研究生
Supported by:
国家自然科学基金资助项目（No.30960448），江西省自然科学基金项目（No.20114BAB205033），江西省教育厅科技项目（No.GJJ11596、GJJ12584）

摘要/Abstract

摘要： 目的：探讨TreadScan步态分析系统在评价大鼠脊髓挫伤（spinal cord injury，SCI）模型中的应用价值。方法：采用连有PinPoint™精密接触传感器的Benchmark^TM立体定位颅脑撞击器制备脊髓挫伤大鼠模型。利用Image J图像分析软件测定伤后30 min损伤中心及其邻近区域的初始损伤体积。应用TreadScan步态分析系统描述术前及伤后7，14，21，28 d大鼠的行为学特征。采用Harris氏HE染色结合Luxol固蓝染色技术观察伤后1，7，14，28 d损伤组织的形态学改变。结果：模型组脊髓初始损伤体积为（4.17 ± 0.1）mm³（X±s, n=5），范围在4.02至4.32 mm³之间，呈近似正态分布。与术前比较，模型组术后各项行为学参数差异均具有显著性（P<0.05）。与假手术组比较，伤后模型组脚印面积、步长、瞬间跑速、正常步序比均下降显著（P<0.05），而跨步时间、摆动时间、后脚脚间距、最小纵向偏差均显著上升（P<0.05）。伤后1 d除损伤中心外，邻近区域组织形态基本完好。但是随时间延长，模型组组织溃变和髓鞘脱失的严重程度显著高于假手术组，且有向周边蔓延趋势。结论：采用连有PinPoint™精密接触传感器的Benchmark^TM立体定位颅脑撞击器制备的脊髓挫伤大鼠模型具有良好的重复性。应用TreadScan步态分析系统能够客观准确的评价大鼠SCI后的运动功能。

关键词: 脊髓挫伤, 大鼠, 初始损伤体积, 行为学评价, 步态分析

Abstract: Objective:To assess the utility of TreadScan gait analysis systemin evaluating rat spinal cord contusion model. Methods: Rat spinal cord contusion model was established by a Benchmark^TM stereotaxic cortical impactor which was connected to PinPoint™ precision contact sensor. Image J image analysis software was used to measure the initial lesion volume of injured epicenter and adjacent area at posttraumatic 30 min. TreadScan gait analysis system was applied to describe the behavioral characteristics of the rats at preoperative and posttraumatic 7, 14, 21, 28 d. Morphological changes were inspected by using the Harris's HE staining in combination with Luxol fast blue staining technique at posttraumatic 1, 7, 14, 28 d. Results: The initial lesion volume of spinal cord in model group was (4.17±0.15) mm³ (±s, n=5) with a range of 4.02 to 4.32 mm³ and fitted to the normal distribution. As compared to the preoperative, there were significant differences (P<0.05) in each behavior parameters of the postoperative in model group. As compared to the sham group, the Average Print Area, Stride Length, Inst Run Speed and Regularity Index were decreased significantly in model group (P<0.05 ). However, the Stride Time, Swing Time, Rear Track Width, Minimum Longitudinal Deviation were significantly increased (P<0.05). In addition to the injured epicenter, the morphology of adjacent region tissue was basically intact at posttraumatic 1 d. However, overtime the severity of tissue degeneration and demyelination in model group were significantly higher than those in sham group, with the trend
of spreading to the surrounding areas. Conclusion: The rat spinal cord contusion model prepared by a Benchmark^TM
stereotaxic cortical impactor has a good replicability. TreadScan gait analysis system was particularly objective and accurate in evaluating the behavioral characteristics after SCI in rats.

Key words: spinal cord contusion, rats, initial lesion volume, behavior evaluation, gait analysis

张思，顾兵，王烁宇，李华南，张国福，张水印 . TreadScan步态分析系统在评价大鼠脊髓挫伤模型中的应用[J]. 神经药理学报, 2012, 2(5): 8-18.

Zhang Si, GU Bing1 WANG Shuo-yu2, LI Hua-nan2, ZHANG Guo-fu, ZHANG Shui-yin1, . Assessment of TreadScan Gait Analysis System in the Evaluation of Rat Spinal Cord Contusion Model[J]. Acta Neuropharmacologica, 2012, 2(5): 8-18.

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