柴胡疏肝散醇提物对抑郁模型小鼠的抗抑郁作用

神经药理学报 ›› 2014, Vol. 4 ›› Issue (5): 8-14.

柴胡疏肝散醇提物对抑郁模型小鼠的抗抑郁作用

于亚青，王林林，王静静，魏丽，张万明

1河北北方学院药学系，张家口，075000，中国 2河北北方学院附属第一医院，张家口，075000，中国

出版日期:2014-10-26 发布日期:2015-01-20
通讯作者: 张万明，教授，硕士生导师，从事药物分析、药理学研究， E-mail：zwm19650228@163.com
作者简介:于亚青，在读硕士研究生，从事药物分析、药理学研究，E-mail：1243591405@qq.com
基金资助:
国家自然科学基金项目（NO.81274005）；河北省教育厅基金资助项目（z2008106）；河北北方学院基金资助项目（2001017）

Antidepressant Effects of Chaihu Shugan Powder Alcohol Extracts in Mice Depression Model

YU Ya-qing,WANG Lin-lin,WEI Li , ZHANG Wan-ming

Department of Pharmanacy, Hebei North university, Zhangjiakou, 075000, China

Online:2014-10-26 Published:2015-01-20
Contact: 张万明，教授，硕士生导师，从事药物分析、药理学研究， E-mail：zwm19650228@163.com
About author:于亚青，在读硕士研究生，从事药物分析、药理学研究，E-mail：1243591405@qq.com
Supported by:
国家自然科学基金项目（NO.81274005）；河北省教育厅基金资助项目（z2008106）；河北北方学院基金资助项目（2001017）

摘要/Abstract

摘要： 目的：研究柴胡疏肝散（Chaihu Shugan Powder，CSGS）乙醇提取物对抑郁模型小鼠行为学的影响。方法：采用慢性不可预见温和应激法（Chronic unpredictable mild stress, CUMS）与慢性束缚应激法（Chronic restraint stress, CRS）相结合复制小鼠抑郁模型，采用体重增长趋势、蔗糖饮水试验、敞箱实验、情绪性行为评分、悬尾实验、强迫游泳实验来评价柴胡疏肝散醇提物抗抑郁作用。结果：CUMS与CRS相结合可以导致小鼠抑郁：与正常组相比，模型组小鼠体重增长趋势明显减慢（P＜0.01）；在蔗糖饮水实验中，模型组小鼠的蔗糖水偏嗜度下降；在敞箱实验中，模型组小鼠水平运动次数和垂直运动次数均显著性下降（P＜0.01，P＜0.05），具有统计学意义；在情绪性行为评分实验中，模型组小鼠情绪评分显著性降低（P＜0.05）；在悬尾实验中，模型组小鼠的悬尾不动时间显著性升高（P＜0.01）；在强迫游泳实验中，模型组小鼠的游泳不动时间显著性升高（P＜0.01）。与模型组相比，柴胡疏肝散水提组和醇提物4.1，8.2，16.4 g·kg-1剂量组均可改善小鼠的抑郁症状（P＜0.05，P＜0.01），且具有剂量相关性。同是8.2g·kg-1的生药量，与水提物比较，醇提物组小鼠实验第28天体重明显高于水提组（P＜0.05），敞箱实验中水平运动次数明显高于水提组（P＜0.05），强迫游泳实验中游泳不动时间明显低于水提组（P＜0.05），具有统计学意义。结论：本实验通过CUMS与CRS相结合方法成功复制了小鼠抑郁模型。行为学实验证实了模型复制成功，同时也表明柴胡疏肝散醇提物具有明显的抗抑郁作用。

关键词: 柴胡疏肝散, 醇提物, 水提物, 抑郁模型, 行为学

Abstract: Objective: To investigate the effects of Chaihu Shugan powder (CSGS) alcohol extracts on behaviors in a mouse model of depression. Methods: Mice model of depressiion was established by combination of CUMS and CRS to evaluate the antidepressant effects by performing the body weight growth rate, sucrose preference test, open field test, emotional behavior test, tail suspension test, and force swimming test. Results: Combation of CUMS and CRS induced significant depressive behaviors as compared with the control group. Namely, the body weight growth rate was slower (P＜0.01); the sucrose preference rate reduced; the acrossing-movement times and rearing-movement times of model mice were lower in open field test (P＜0.01, P＜0.05); the emotional behavior score of model mice was lower in emotional behavior test (P＜0.05); the non-movement time of model mice was longer in tail suspension test (P＜0.01); the immobility time was increased in force swimming test of model mice (P＜0.01). Compared with the saline group, treatment of alcohol extract of Chaihu Shugan powder (4.1, 8.2, 16.4 g·kg-1) could improve the symptoms of depressive mice in a dose-related manner (P＜0.01, P＜0.05). Based on the equal amount of crude drug (8.2 g·kg-1), treatment of alcohol extract for 28 days showed higher body weight and acrossing-movement times, and lower immobility time in the open field test and force swimming test respectively (P＜0.05). Conclusion: Depression model was successfully established by combining the CUMS and CRS. CSGS alcohol extract showed notable antidepressant effects.

Key words: Chaihu Shugan Powder, alcohol extracts, water extracts, depression model, ethology

中图分类号:

R965

于亚青, 王林林, 王静静, 魏丽, 张万明. 柴胡疏肝散醇提物对抑郁模型小鼠的抗抑郁作用[J]. 神经药理学报, 2014, 4(5): 8-14.

YU Ya-qing, WANG Lin-lin, WEI Li, ZHANG Wan-ming. Antidepressant Effects of Chaihu Shugan Powder Alcohol Extracts in Mice Depression Model[J]. ACTA NEUROPHARMACOLOGICA, 2014, 4(5): 8-14.

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