关键词: 均匀设计-高通量筛选, 丹参, 水溶性成分, 配比

Abstract: Objective: To evaluate the application of uniform design-high throughput screening technology in the study of the active ingredient of traditional Chinese medicine, vascular endothelial cells protective effect is detected by multifactorand multilevel and uniform design-high throughput screening method of Radix Salviae Miltiorrhizae hydrosoluble constituents. Methods: We performed uniform design compatibility study on seven Radix Salviae Miltiorrhizae hydrosoluble constitutents, each of which at seven concentration levels (1×10-4～1×10-10mol·L-1) and their protective effect is assessed by the damage of vascular endothelial cells injured by H2O2 and serum deprivation. Results: After high-throughput screening and rescreening, the best  Radix Salviae Miltiorrhizae hydrosoluble combination were obtained with A2 [cell survival ratio is (70±4)%] and B4 [cell survival ratio is (76±3)%]. Different combination samples were identified with different vascular endothelial cells model which was damaged by H2O2 and serum deprivation . Conclusion: Uniform design compatibility and the traditional Chinese medicine compound compatibility have similarities．Uniform design-high throughput screening technology is a reliable technology for massive potency screening of active ingredients of traditional Chinese medicine and compounds.

Key words: uniform design-high throughputscreening, Radix Salviae Miltiorrhizae, hydrosoluble constituents, proportion