神经药理学报

神经药理学报 ›› 2014, Vol. 4 ›› Issue (1): 17-19.

• 研究论文 • 上一篇    下一篇

和营安心方对过氧化氢损伤大鼠胚胎心肌细胞的保护作用

贺 伟， 李永民， 张海霞， 朱 娟， 高 月   

  1. 河北北方学院， 张家口，075000，中国
  • 出版日期:2014-02-26 发布日期:2014-06-06
  • 通讯作者: 李永民，男，教授，博士，硕士生导师；研究方向：中药药理学；Tel：+86-0313-4029228，E-mail: liyongmin2001@sina.com
  • 作者简介:贺伟，女，助教，硕士；研究方向：中药药理学；Tel：+86-0313-4029221；E-mail：heweihebei@163.com
  • 基金资助:

    河北省教育厅高等学校科学研究课题（No. 2010215）

Protective Effects of He Ying An Xin Fang on Free Radical Injury Induced by H2O2 in Myocardium Rats

HE Wei, LI Yong-min, ZHANG Hai-xia, ZHU Juan, GAO Yue   

  1. Hebei North University, Zhangjiakou, 075000, China
  • Online:2014-02-26 Published:2014-06-06
  • Contact: 李永民，男，教授，博士，硕士生导师；研究方向：中药药理学；Tel：+86-0313-4029228，E-mail: liyongmin2001@sina.com
  • About author:贺伟，女，助教，硕士；研究方向：中药药理学；Tel：+86-0313-4029221；E-mail：heweihebei@163.com
  • Supported by:

    河北省教育厅高等学校科学研究课题（No. 2010215）

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摘要： 目的：研究和营安心方对过氧化氢（hydrogen peroxide，H2O2）所致大鼠胚胎心肌细胞损伤的保护作用。方法：提取和营安心方，采用H2O2建立大鼠胚胎心肌细胞氧化应激损伤模型。实验分为5组，分别为：正常对照组（Control）、过氧化氢（200μmol·L-1）模型组（Model）、和营安心方（500，250，125mg·kg-1）三个剂量组。通过四甲基偶氮唑盐（3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl tetrazolium bromide，MTT）代谢率检测心肌细胞的细胞活性，并检测细胞培养液中乳酸脱氢酶（lactate dehydrogenase，LDH）及丙二醛（malondialdehyde，MDA）的含量。结果：H2O2组细胞活性显著性降低（P＜0.01），LDH及MDA含量显著性升高（P＜0.01）；和营安心方可显著提高H2O2损伤的心肌细胞活性，减少MDA 含量（P＜0.05），降低LDH活性（P＜0.05）。结论：和营安心方对H2O2损伤的心肌细胞具有明显的保护作用，其机制可能与抑制脂质过氧化有关。

关键词: 和营安心方, 过氧化氢, 心肌细胞, 氧化应激

Abstract: Objective:To investigate the protective effects and mechanisms of he ying an xin fangon myocardium injury caused by H2O2. Methods: Hydrogen peroxide (H2O2) was adopted to establish the rat embryonic cardiomyocytes oxidative stress insult model , and the experiment was divided into 5 groups: cardiomyocytes were incubated by H2O2 (200 μmol·L-1) for 2h as model group, the non- H2O2 as control group, and the extraction of he ying an xin fang (500, 250, 125 mg·kg-1) as therapeutic groups.
The cell viabilityby 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), content of malondialdehyde (MDA) and activity of lactate dehydrogenase (LDH) were measured. Resulds: Cell viabilityby MTT: compared with control group, cell Viabilityby MTT was decreased significantly in H2O2 group (P< 0.01), while he ying an xin fang had no significant effect on cardiac myocytes of cell viability. Content of MDA: compared with that of H2O2 group, the content of MDA was decreased in he ying an xin fang groups significantly (P< 0.05). Content of LDH: compared with that of control group, the content of LDH was decreased in he ying an xin fang groups significantly (P< 0.05). Conclusion: He
ying an xin fang has significant protective effects on free radical injury induced by H2O2 in myocardium rats, and it may be related to the inhibition of lipid peroxidation.

Key words: He ying an xin fang, hydrogen peroxide (H2O2), myocardium, oxidative
stress