甲氨蝶呤治疗大鼠脊髓挫伤对急性期脂质过氧化的影响

神经药理学报 ›› 2014, Vol. 4 ›› Issue (1): 11-16.

甲氨蝶呤治疗大鼠脊髓挫伤对急性期脂质过氧化的影响

张思¹ ，顾兵^1 ，王烁宇^2 ，李华南² ，张国福² ，张水印¹

1 江西科技师范大学生命科学学院，南昌，330013，中国
2 江西中医药大学附属医院脊柱外科，南昌，330006，中国

出版日期:2014-02-26 发布日期:2014-06-06
通讯作者: 顾兵，男，博士后，教授，硕士生导师；研究方向：神经精神药物学；E-mail: bguemory@hotmail.com
作者简介:张思，女，硕士生；研究方向：化学生物学；E-mail: 847404553 @qq.com
基金资助:
国家自然科学基金项目（No.30960448），江西省自然科学基金项目（No.20114BAB205033），江西省教育厅科技项目（No.GJJ11596、GJJ12584）

Effects of Methotrexate on Acute Phrase of Spinal Cord Contusion-Induced Lipid Peroxidation in Rats

ZHANG Si¹, GU Bing¹, WANG Shuo-yu², LI Hua-nan², ZHANG Guo-fu², ZHANG Shui-yin¹

1. College of Life Science, Jiangxi Science & Technology Normal University, Nanchang, 330013, China 2. Department of Spine Surgery, The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, China

Online:2014-02-26 Published:2014-06-06
Contact: 顾兵，男，博士后，教授，硕士生导师；研究方向：神经精神药物学；E-mail: bguemory@hotmail.com
About author:张思，女，硕士生；研究方向：化学生物学；E-mail: 847404553 @qq.com
Supported by:
国家自然科学基金项目（No.30960448），江西省自然科学基金项目（No.20114BAB205033），江西省教育厅科技项目（No.GJJ11596、GJJ12584）

摘要/Abstract

摘要： 目的：观察甲氨蝶呤对大鼠脊髓挫伤后脂质过氧化的影响，探讨其急性期抗氧化神经保护机制。方法：采用PinPoint^TM精密皮质撞击器制备大鼠脊髓挫伤模型。伤后30 min皮下注射甲氨蝶呤（0.5 mg·kg^-1·BW）。采用酶联免疫吸附法检测血浆中丙二醛（malondialdehyde，MDA）和8-异前列腺素F_2α（8-iso-prostaglandin F_2α，8-iso-PGF_2α）的含量以及损伤组织中4-羟基壬烯醛-His加合物（4-hydroxynonenal-His adduct，HNE）的含量。结果：伤后1，3，6，12，24，48，72 h，甲氨蝶呤组MDA、8-iso-PGF_2α以及HNE的含量均低于模型组。尤其在伤后6，12 h，甲氨蝶呤组血浆中MDA和脊髓组织中HNE含量均显著低于模型组（P<0.05）；在伤后12 h，血浆中8-iso-PGF_2α含量也显著低于模型组（P<0.05）。结论：合适剂量的甲氨蝶呤防止脊髓继发性损伤可能与抑制或减轻脂质过氧化有关。

关键词: 甲氨蝶呤, 创伤性脊髓损伤, 急性期, 脂质过氧化, 酶联免疫吸附法

Abstract: Objective：This study examined the effect of methotrexate on acute phrase of spinal cord contusion-induced lipid peroxidation in rats and the neuroprotective mechanism of its anti-oxidant action. Methods: Rat spinal cord contusion model was prepared by Pinpoint Precision Cortical Impactor™ apparatus and then methotrexate (0.5mg•kg^-1•BW)
was subcutaneously administrated at posttraumatic 30 min. ELISA method was used to determine the content of malondialdehyde（MDA）and 8-iso-PGF_2αin plasma and 4-hydroxynonenal-His adduct (HNE) in injury tissue. Results: Posttraumatic 1, 3, 6, 12, 24, 48, 72 h, the contents of MDA, 8-iso-PGF_2αand HNE in the methotrexate group were all lower than those in the model group, which achieved statistical significance at posttraumatic 6, 12 h (P<0.05). At posttraumatic 12 h, the content of 8-iso-PGF2α in plasma was also significantly lower than that in the model group (P<0.05). Conclusion: The appropriate doses, methotrexate could prevent secondary injury of spinal cord, which may be related to inhibiting or reducing the lipid peroxidation.

Key words: methotrexate, traumatic spinal cord injury, acute phase, lipid peroxidation, enzyme linked immunosorbent assay

张思，顾兵，王烁宇，李华南，张国福，张水印. 甲氨蝶呤治疗大鼠脊髓挫伤对急性期脂质过氧化的影响[J]. 神经药理学报, 2014, 4(1): 11-16.

ZHANG Si, GU Bing, WANG Shuo-yu, LI Hua-nan, ZHANG Guo-fu, ZHANG Shui-yin. Effects of Methotrexate on Acute Phrase of Spinal Cord Contusion-Induced Lipid Peroxidation in Rats[J]. Acta Neuropharmacologica, 2014, 4(1): 11-16.

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