神经药理学报

神经药理学报 ›› 2013, Vol. 3 ›› Issue (4): 8-13.

• 研究论文 • 上一篇    下一篇

大黄素对脑缺血-再灌注小鼠探索认知功能的改善作用

王树, 张海红, 薛贵平   

  1. 河北北方学院药学系，张家口，075000，中国
  • 出版日期:2013-08-26 发布日期:2014-06-27
  • 作者简介:王树，男，副教授，硕士；研究方向：神经药理学；Tel: +86-313-4029308, E-mail: wangshu388@163.com
  • 基金资助:

    河北省自然科学基金项目(No. C2009001026)，河北省科技攻关计划项目（No.07276166），河北省教育厅项目(No.2010212)

Effects of Emodin on Exploratory and Cognitive Function of Mice Experiencing Cerebral Ischemia Reperfusion

WANG Shu, ZHANG Hai-hong, XUE Gui-ping   

  1. Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China
  • Online:2013-08-26 Published:2014-06-27
  • About author:王树，男，副教授，硕士；研究方向：神经药理学；Tel: +86-313-4029308, E-mail: wangshu388@163.com
  • Supported by:

    河北省自然科学基金项目(No. C2009001026)，河北省科技攻关计划项目（No.07276166），河北省教育厅项目(No.2010212)

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摘要： 目的：研究大黄素对脑缺血再灌注小鼠探索认知功能的改善作用及其机制。 方法：采用改进的Himori法暂时性阻断两侧颈总动脉制备小鼠脑缺血再灌注损伤模型，进行探索实验和跳台实验，观察大黄素腹腔注射（intraperitoneal injection, ip）（10.0, 1.0, 0.1 mg·kg-1）对脑缺血再灌注小鼠探索认知功能的改善作用，并对各剂量组小鼠脑组织和血液中一氧化氮（nitric oxide，NO）含量和一氧化氮合酶（nitric oxide synthase，NOS）活力、脑组织中过氧化氢（hydrogen peroxide，H2O2）含量和过氧化氢酶（catalase，CAT）活力进行测定，并测定脑指数。 结果：大黄素可改善脑缺血再灌注损伤所致的探索认知功能障碍；减少NO和H2O2含量， 降低NOS活力，提高CAT活力和增加脑指数。结论：大黄素对脑缺血再灌注损伤小鼠探索认知功能有改善作用，其作用机制可能是通过降低NOS活力和增强CAT活力，提高脑组织对氧自由基的清除能力，从而减轻缺血再灌注引起的脑组织损伤。

关键词: 大黄素, 脑缺血再灌注, 学习记忆, 认知功能, 探索实验

Abstract: Objective: To study the effects and mechanisms of emodin on exploratory and cognitive functions of mice experiencing cerebral ischemia reperfusion. Methods: Using improved Himori method, cerebral ischemia reperfusion injury was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. Effects of emodin (10.0, 1.0, 0.1 mg.kg-1，ip) on exploratory and cognitive function of mice experiencing cerebral ischemia reperfusion were assessed using the exploratory movement test and the step-through and step-down tests. The content of nitric oxide (NO) and the nitric oxide synthase (NOS) activities in brain and plasma, the content of hydrogen peroxide (H2O2) and catalase (CAT) activities in brain, and brain index were also measured. Results: Emodin significantly improved the exploratory and cognitive impairment induced by cerebral ischemia reperfusion injury, reduced the content of NO and H2O2, decreased the activities of NOS, and increased the activities of CAT and brain index. Conclusions: Emodin improved the performance in tests assessing exploratory and cognitive functions in mice experiencing cerebral ischemia reperfusion injury. Possible mechanisms include the reduction of the activities of NOS and enhancement of the activities of CAT, and clearance of the oxygen free radicals.

Key words: emodin, cerebral ischemia reperfusion, learning and memory, cognitive function, exploratory movement test

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