他莫昔芬对雌性大鼠学习记忆能力的影响

神经药理学报 ›› 2013, Vol. 3 ›› Issue (3): 1-7.

• 研究论文 • 下一篇

他莫昔芬对雌性大鼠学习记忆能力的影响

李增芳^1,2 ，贺晓丽² ，毕明刚²

1.河北北方学院，张家口，075000，中国
2.中国医学科学院药用植物研究所，北京，100193，中国

出版日期:2013-06-26 发布日期:2014-06-27
通讯作者: 毕明刚，男，博士，研究员，博士生导师；Tel: +86-010-57833233，E-mail: mgbi@implad.ac.cn
作者简介:李增芳，女，硕士；研究方向：神经药理；E-mail: zengfangtiankong@163.com
基金资助:
“重大新药创制”科技重大专项（No.2012ZX09301002-001-026), 国家自然科学基金青年科学基金项目（No.81202192）

Effects of Tamoxifen on Learning and Memory in Female Rats

LI Zeng-fang 1,2，HE Xiao-li 2，BI Ming-gang 2

1. Hebei North University, Zhangjiakou, 075000, China
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, 100193, China

Online:2013-06-26 Published:2014-06-27
Contact: 毕明刚，男，博士，研究员，博士生导师；Tel: +86-010-57833233，E-mail: mgbi@implad.ac.cn
About author:李增芳，女，硕士；研究方向：神经药理；E-mail: zengfangtiankong@163.com
Supported by:
“重大新药创制”科技重大专项（No.2012ZX09301002-001-026), 国家自然科学基金青年科学基金项目（No.81202192）

摘要/Abstract

摘要： 目的：探讨腹腔注射选择性雌激素受体调节剂他莫昔芬（tamoxifen, TC）对雌性大鼠学习记忆能力的影响。方法：将60只成年雌性SD大鼠随机分为空白对照组、溶剂对照组、TC 2.5 mg•kg-1组、TC 5 mg•kg-1组、TC 10 mg•kg-1组5组，采用Morris水迷宫、跳台及八臂迷宫等行为学实验检测TC对雌性SD大鼠空间辨别学习记忆能力、被动回避条件反射能力及主动学习记忆能力的影响。结果：与溶剂对照组相比，3周后，TC各剂量组被动回避条件反射能力明显下降（P<0.05），表现为跳台实验中的逃避潜伏期显著减少，错误反应次数明显增加。6周后，TC各剂量组大鼠的空间辨别学习记忆能力、被动回避条件反射能力及主动学习记忆能力均明显下降（P<0.05），表现为Morris实验定位航行的潜伏期增加，目的象限的停留时间减少，第一次到达平台的时间延长；跳台实验中逃避潜伏期显著减少，错误反应次数明显增加；八臂迷宫实验中主动学习过程中总路程明显延长，典型性错误次数显著增加。结论：雌性SD大鼠腹腔注射TC 6周后，其学习记忆能力随着TC剂量的增加而降低，TC对雌性SD大鼠的学习记忆能力具有明显的损伤作用。

关键词: 他莫昔芬, 学习记忆, 雌性, 大鼠, 选择性雌激素受体调节剂

Abstract: Objective: To investigate the effects of tamoxifen on learning and memory abilities in female rats after intraperitoneal (i.p.) injection of the selective estrogen receptor modulator tamoxifen. Methods: Adult female Sprague-Dawley (SD) rats (n=60) were randomly assigned into 5 groups (control group, vehicle group, TC 2.5 mg•kg-1 group, TC 5 mg•kg-1 group and TC 10 mg•kg-1 group). Morris water maze test, step-down test and 8-arm maze task were used to test the effects of tamoxifen on spatial learning and memory ability, passive avoidance conditioned reflex ability and active learning and memory ability in female SD rats, respectively. Results: Compared with vehicle group, passive avoidance conditioned reflex ability of the rats after i.p. injection of TC for three weeks was significantly decreased (P<0.05). After six weeks of drug treatment, the discriminability on spatial learning and memory ability, passive avoidance conditioned reflex ability and active learning and memory ability were all significantly reduced in these female SD rats (P<0.05). Conclusion: The results suggested that after tamoxifen treatment for six weeks, the learning and memory abilities in female SD rats were dose-dependently decreased, and that tamoxifen could cause substantial learning and memory deficits in female SD rats.

Key words: tamoxifen, learning and memory, female, rats, selective estrogen receptor modulators

中图分类号:

R965

李增芳，贺晓丽，毕明刚 . 他莫昔芬对雌性大鼠学习记忆能力的影响[J]. 神经药理学报, 2013, 3(3): 1-7.

LI Zeng-fang,HE Xiao-li,BI Ming-gang. Effects of Tamoxifen on Learning and Memory in Female Rats[J]. Acta Neuropharmacologica, 2013, 3(3): 1-7.

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他莫昔芬对雌性大鼠学习记忆能力的影响

Effects of Tamoxifen on Learning and Memory in Female Rats

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