蛇床子素对AD大鼠神经元凋亡及细胞周期的影响

神经药理学报 ›› 2012, Vol. 2 ›› Issue (3): 7-14.

蛇床子素对AD大鼠神经元凋亡及细胞周期的影响

董晓华¹，孟宪勇²，张力¹，杨建明¹

1. 河北北方学院药学系，张家口，075000，中国
2. 河北北方学院附属第一医院，张家口，075000，中国

出版日期:2012-06-26 发布日期:2013-12-25
通讯作者: 张力，男，教授，硕士生导师；研究方向：神经药理学；Tel：+86-0313-4029188，E-mail：zmczl@hotmail.com
作者简介:董晓华，女，副教授，博士；研究方向：神经药理学；Tel：+86-0313-4029305，E-mail：hbdxh76@163.com
基金资助:
河北省卫生厅资助项目(No.20090183)，河北省科技厅资助项目（No.13272704D）

Effects of Osthole on Neuronal Apoptosis and Cell Cycle in AD Rats

DONG Xiao-hua¹, MENG Xian-yong², ZHANG Li¹ , YANG Jian-ming¹

1. Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China 2. The First Attached Hospital, Hebei North University, Zhangjiakou, 075000, China

Online:2012-06-26 Published:2013-12-25
Contact: 张力，男，教授，硕士生导师；研究方向：神经药理学；Tel：+86-0313-4029188，E-mail：zmczl@hotmail.com
About author:董晓华，女，副教授，博士；研究方向：神经药理学；Tel：+86-0313-4029305，E-mail：hbdxh76@163.com
Supported by:
河北省卫生厅资助项目(No.20090183)，河北省科技厅资助项目（No.13272704D）

摘要/Abstract

摘要： 目的：观察蛇床子素对阿尔茨海默病(Alzheimer's disease, AD)模型大鼠神经元凋亡及细胞周期的影响，探讨蛇床子素的神经保护作用及其作用机制。方法：采用一次性侧脑室注射聚集态β淀粉样肽(β-amyloid peptide，Aβ_25-35)建立AD大鼠模型，腹腔注射12.5，25.0 mg·kg^-1蛇床子素进行干预，观察大鼠认知功能、神经元凋亡及细胞周期变化。结果：蛇床子素能明显改善AD模型大鼠空间学习记忆能力，减少神经元凋亡，增加S期细胞百分率，促进G₂/M期细胞进一步分裂，增强细胞增殖活性，调节细胞周期，有利于维持神经元正常的生理功能。结论：蛇床子素可通过减少神经元凋亡、调节细胞周期，具有神经保护作用，这可能是其改善AD大鼠学习记忆障碍的作用机制之一。

关键词: 蛇床子素, 阿尔茨海默病, 学习记忆, 神经元凋亡, 细胞周期

Abstract: Objective: To observe the influence of osthole on neuronal apoptosis and cell cycle in Alzheimer's disease (AD) rats and to study the neuroprotective effects and its mechanism. Methods: An intracerebroventricular (i.c.v.) injection of β-amyloid peptide (Aβ_25-35) was administrated to establish AD rat model. Osthole(12.5，25.0 mg·kg^-1) was injected intraperitoneally to rats and cognitive functions, neuronal apoptosis and cell cycle of AD rats were observed. Results: Osthole could improve spatial learning and memory abilities of AD rats, reduce neuronal apoptosis, increase the percentage of S phase cells, promote the G₂/M phase cells to divide further, strengthen the cell proliferation activity, regulate cell cycle and benefit to the maintenance of normal physiological function of neurons. Conclusion: Osthole hasneuronal protection through reducing neuronal apoptosis and regulating cell cycle, which may be one of mechanism of improving learning and memory disorder in AD rats.

Key words: osthole, Alzheimer&, rsquo, s disease, learning and memory, neuronal apoptosis, cell cycle

中图分类号:

R965

董晓华，孟宪勇，张力，杨建明. 蛇床子素对AD大鼠神经元凋亡及细胞周期的影响[J]. 神经药理学报, 2012, 2(3): 7-14.

DONG Xiao-hua, MENG Xian-yong, ZHANG Li, YANG Jian-ming. Effects of Osthole on Neuronal Apoptosis and Cell Cycle in AD Rats[J]. Acta Neuropharmacologica, 2012, 2(3): 7-14.

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