神经药理学报

神经药理学报 ›› 2011, Vol. 1 ›› Issue (5): 14-18.

• 研究论文 • 上一篇    下一篇

注射用地龙冻干粉针对大鼠颈动脉血栓形成的影响

梁超1，刘冬洁2，高瑞辰3，韩丽敏3，曲轶3，沈鑫3，朱久新3，侯云龙3，乔国芬3
   

  1. 1. 哈尔滨医科大学附属第一医院，黑龙江，150081，中国
    2. 大庆油田总医院肿瘤科，黑龙江，163000，中国
    3. 哈尔滨医科大学药理教研室，黑龙江，150081，中国
  • 出版日期:2011-10-26 发布日期:2013-03-24
  • 通讯作者: 乔国芬，女，教授；Tel：+86-0451-86671354，E-mail：qiaogf88@yahoo.com.cn
  • 作者简介:梁超，男，副主任药师；E-mail：haerbinliangchao@126.com.
  • 基金资助:

    黑龙江省教育厅科学技术研究项目（No.L12511320），哈尔滨医科大学大学生创新创业训练计划

Anti-thrombotic Effect of Earthworm Lyophilized Powder for Injection

LIANG Chao1, LIU Dong-jie2, GAO Rui-chen3, HAN Li-min3, QU Yi3, SHEN Xin3, ZHU Jiu-xin3, HOUYun-long3, QIAO Guo-fen3   

  1. 1. The First Affiliated Hospital of Harbin Medical University, Harbin, 150081, China
    2. Department of Oncology, the General Hospital of Daqing Oil Field, Daqing, 163000, China
    3. Department of Pharmacology, Harbin Medical University, Harbin, 150081, China
  • Online:2011-10-26 Published:2013-03-24
  • Contact: 乔国芬，女，教授；Tel：+86-0451-86671354，E-mail：qiaogf88@yahoo.com.cn
  • About author:梁超，男，副主任药师；E-mail：haerbinliangchao@126.com.
  • Supported by:

    黑龙江省教育厅科学技术研究项目（No.L12511320），哈尔滨医科大学大学生创新创业训练计划

PDF (PC)

2409

被引次数

6

摘要： 目的：评价注射用地龙冻干粉针的抗血栓作用。方法：SD雄性大鼠40只，利用血栓生成仪复制颈动脉血栓模型，模型成功后72h，将动物随机分成空白组、地龙冻干粉针1.8，0.9mg•kg-1剂量组及阳性药疏血通注射液对照组，每组10只，各组大鼠分别尾静脉注射(iv)生理盐水、地龙冻干粉针1.8，0.9mg•kg-1剂量溶液和疏血通注射液(0.1mL•kg-1)12天后，剥离颈动脉血栓，测定血栓重量，并对各组大鼠腹主动脉取血，利用ELISA法测定血浆组织型纤溶酶原激活剂(tissue type plasminogen activator，t-PA)含量，采用观察法测定各组大鼠的凝血时间（clotting time，CT）、血浆复钙时间（recalcification time，RT），利用血小板聚集仪测定血小板最大聚集率。结果：与空白组相比较，地龙冻干粉针1.8mg•kg-1剂量组大鼠血栓重量显著减少（P<0.05）；阳性药疏血通组与地龙冻干粉针0.9mg•kg-1剂量组大鼠血浆t-PA含量显著升高（P<0.05）；阳性药疏血通组、地龙冻干粉针1.8mg•kg-1剂量组大鼠CT、RT显著延长（P<0.05），0.9mg•kg-1剂量组RT显著延长（P<0.05），且地龙冻干粉针对RT延长呈剂量依赖性。结论：注射用地龙冻干粉针具有显著的溶解血栓的作用，并能够抑制内源性凝血途径，溶血栓作用可能与提高血浆t-PA含量有关。

关键词: 地龙冻干粉针, 颈动脉血栓形成, 抗血栓

Abstract: Objective: To observe the anti-thrombus effects of Earthworm lyophilized powder for injection(ELPI). Methods: The SD rats were randomly divided into blank control, low dose ELPI, and high dose ELPI groups, as well as ShuXueTong injection as the positive control. The models of carotid artery thrombus were induced by thrombus formation instrument; 72 hours after successful model establishment saline solution (2 mL•kg-1), earthworm low dose solution (0.9 mg•kg-1), earthworm high dose solution (1.8 mg•kg-1), and ShuXueTong solution (0.1 mL•kg-1) were injected via tail vein for 12 consecutive days. Then the carotid artery thrombus were peeled off and weighed. Blood was sampled for plasma t-PA level determination via ELISA method, CT and RT values were measured in all groups, and the maximal platelet aggregation rate was (PagR-max) was tested via LBY-NJ4 method. Results: Compared with the control group, thrombus weights in earthworm high dose group decreased significantly (P < 0.05); plasma t-PA levels in both low dose and positive control groups were significantly improved (P < 0.05); CT and RT in positive control and high-dose groups were significantly prolonged (P < 0.05), RT in low dose group also prolonged (P < 0.05), and this RT prolongation induced by ELPI was dose-dependent. Conclusion: ELPI possesses significant thrombolytic effects after 72 hours administration, and can inhibit intrinsic coagulation pathway through increasing plasma level of t-PA.

Key words: Earthworm lyophilized powder for injection, carotid artery thrombosis, anti-thrombosis

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