棉籽总黄酮抗抑郁活性及其对海马单胺水平的调节

神经药理学报 ›› 2011, Vol. 1 ›› Issue (4): 6-11.

棉籽总黄酮抗抑郁活性及其对海马单胺水平的调节

赵楠¹，张有志¹，杨明¹，王伊文²，赵毅民¹，宫泽辉¹，李云峰¹

1.军事医学科学院毒物药物研究所，北京，100850，中国
2.解放军302医院药学部，北京，100039，中国

出版日期:2011-08-26 发布日期:2012-11-24
通讯作者: 李云峰，男，博士，研究员，博士生导师；研究方向：精神药理学；Tel：+86-010-66874606，E-mail：Lyf619@yahoo.com.cn
作者简介:赵楠，女，硕士，实验师；研究方向：精神药理学；Tel：+86-010-66874606，E-mail：zhaonanzhn@yahoo.com.cn
基金资助:
北京市自然科学基金项目（No.7042052），国家高技术研究发展计划（863计划）资助项目（No.2002AA2Z3131）

The Antidepressant Effect of the Total Flavonoids Extracted fromCottonseed and its Regulation on Hippocampal Monoamines

ZHAO Nan¹，ZHANG You-zhi¹，YANG Ming¹，WANG Yi-wen²，ZHAO Yi-min¹，GONG Ze-hui¹，LI Yun-feng¹

1. Institute of Pharmacology and Toxicology，Academy of Military Medical Sciences，Beijing，100850，China 2. Department of Pharmacy，302 Hospital of PLA，Beijing，100039，China

Online:2011-08-26 Published:2012-11-24

摘要/Abstract

摘要： 目的：探讨棉籽总黄酮（total flavonoids extracted from cottonseed，CTN-T）的抗抑郁作用及可能的作用机制。方法：在大鼠获得性无助模型上评价CTN-T的抗抑郁作用；采用5-羟色氨酸（5-hydroxytryptophan，5-HTP）诱导小鼠甩头实验和育亨宾小鼠毒性增强实验分别观察CTN-T对中枢5-羟色胺（5-hydroxytryptamine，5-HT）和去甲肾上腺素（norepinephrine，NE）能神经系统功能的影响；采用高效液相色谱加电化学检测法测定大鼠海马单胺递质及其代谢产物含量。结果：在大鼠获得性无助模型上，CTN-T（50，100 mg·kg^-1，连续4 d，2次·d^-1，ig）显著减少不可逃避性电击诱导的无助行为；CTN-T（50，75 mg·kg^-1，连续 4 d，2次·d^-1，ig）对 5-HTP 诱导的小鼠甩头行为具有显著增强效应；CTN-T（60 mg·kg^-1，连续 4 d，2次·d^-1，ig）对育亨宾引起的小鼠毒性作用有增强趋势，但差异无统计学意义；大鼠给予 CTN-T（连续 4 d，2次·d^-1，ig）在 25，100 mg·kg^-1剂量下显著升高海马 5-HT含量，在 50，100 mg·kg^-1剂量下显著升高海马 NE含量，在 25~100 mg·kg^-1剂量下对海马多巴胺（dopamine，DA）、肾上腺素（epinephrine，E）、5-羟吲哚乙酸（5-hydroxyindolacetic acid，5-HIAA）含量以及5-HT转换率（5-HT与5-HIAA的比值）无明显影响。结论：CTN-T在大鼠获得性无助模型上具有抗抑郁作用，其作用机制与增强海马 5-HT和 NE能神经功能有关。

关键词: , 抑郁, 棉籽总黄酮, 获得性无助, 5-羟色胺, 去甲肾上腺素

Abstract: Objective：To explore the antidepressant effect of the total flavonoids extracted from cottonseed (CTN-T) and its possible mechanism. Methods：The learned helplessness model in rats was used to evaluate the antidepressant effect of CTN-T. Using the 5-hydroxytryptophan（5-HTP）induced head twitch response test and yohimbine toxicity potentiation test in mice，the possible monoamine targets of the antidepressant action of CTN-T were detected. Moreover， high performance liquid chromatography and electrochemical detection (HPLC-ECD) was used to determine the content of monoamine and metabolites in the hippocampus of rats following CTN-T administration. Results：Repeated administration of CTN-T (50，100 mg·kg^-1，ig，twiceper day for 4 d) significantly decreased the helplessness behavior induced by inescapable foot- shock in learned helplessness test in rats. Repeated administration of CTN-T (50，75 mg·kg^-1， ig，twice per day for 4 d) significantly increased the 5-HTP induced head twitch response in mice，while CTN-T (60 mg·kg^-1，ig，twice per day for 4 d) had a tendency to potentiate yohimbine toxicity in mice. Repeated administration of CTN-T (25~75 mg·kg^-1，ig，twice per day for 4 d)，the neurochemical assays showed that the content of 5-hydroxytryptamine (5-HT) was increased in rat hippocampus at 25 and 100 mg·kg^-1 dose of CTN-T，and that the content of norepinephrine (NE) was increased at 50 and 100 mg·kg^-1 dose of CTN-T. By contrast，CTN-T had no effects on the content of dopamine (DA)，epinephrine (E)，5-hydroxyindolacetic acid (5-HIAA) and the rate of 5-HT/5-HIAA. Conclusion：CTN-T exerts antidepressant effects which may be closely related to the strengthening of the hippocampal serotonergic and noradrenergic function.

Key words: depression, total flavonoids extracted from cottonseed（CTN-T）, learned helplessness model, 5-hydroxytryptamine（5-HT）, norepinephrine（NE）

中图分类号:

R971
R749.42

赵楠，张有志，杨明，王伊文，赵毅民，宫泽辉，李云峰. 棉籽总黄酮抗抑郁活性及其对海马单胺水平的调节[J]. 神经药理学报, 2011, 1(4): 6-11.

ZHAO Nan，ZHANG You-zhi，YANG Ming，WANG Yi-wen，ZHAO Yi-min，GONG Ze-hui，LI Yun-feng. The Antidepressant Effect of the Total Flavonoids Extracted fromCottonseed and its Regulation on Hippocampal Monoamines[J]. Acta Neuropharmacologica, 2011, 1(4): 6-11.

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