神经药理学报

神经药理学报

• 研究论文 • 上一篇    下一篇

DMSO 联合神经营养因子BDNF 诱导BMSCs 向神经样细胞分化

倪爽，苗泽远，王家昕，等   

  1. 1. 河北北方学院基础医学院麻醉专业，张家口，075000，中国
    2. 河北北方学院基础医学院临床专业，张家口，075000，中国
    3. 河北北方学院基础医学院口腔专业，张家口，075000，中国
    4. 河北北方学院基础医学院生物教研室，张家口，075000，中国
    5. 河北北方学院学报编辑部，张家口，075000，中国
  • 出版日期:2019-10-26 发布日期:2020-03-03
  • 通讯作者: 苏立宁，讲师；研究方向：细胞生物学；E-mail：343376274@qq.com
  • 作者简介:倪爽，本科在校生；研究方向：医学麻醉
  • 基金资助:
    2018 年国家级大学生创新创业训练计划项目（No.201810092001），河北北方学院青年基金项目（No.QN2018022）

Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF

NI Shuang，MIAO Ze-yuan，WANG Jia-xin，et al   

  1. 1. Anesthesia Major, Basic Medical College of Hebei North University，Zhangjiakou，075000, China
    2. Clinical Speciality, Basic Medical College of Hebei North University, Zhangjiakou，075000, China
    3. Stomatology, Basic Medical College of Hebei North University, Zhangjiakou，075000, China
    4. Department of Biology, Basic Medical College of Hebei North University, Zhangjiakou, 075000, China
    5. Editorial Department of Journal of Hebei North University，Zhangjiakou，075000，China
  • Online:2019-10-26 Published:2020-03-03
  • Contact: 苏立宁，讲师；研究方向：细胞生物学；E-mail：343376274@qq.com
  • About author:倪爽，本科在校生；研究方向：医学麻醉
  • Supported by:
    2018 年国家级大学生创新创业训练计划项目（No.201810092001），河北北方学院青年基金项目（No.QN2018022）

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摘要： 目的：探讨脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）联合二甲基亚砜（dimethyl sulfoxide，DMSO）诱导骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）向神经样细胞分化的效果，初步检测miRNA-26a 在神经细胞分化过程中的表达量变化。方法：从大鼠股骨抽取骨髓分离BMSCs，DMSO 对其预诱导，随后用含BDNF 的神经细胞培养基培养BMSCs，诱导其分化为神经细胞。免疫组化鉴定分化神经元样细胞特异标记物：神经元特异烯醇酶（neuron specifi c enolase，NSE）。实时定量PCR 法检测神经分化前后酪氨酸磷酸酶蛋白（tyrosine phosphatase protein，PTEN）基因/mTOR 和miRNA-26a 的表达变化。结果：100 ng·mL-1 BDNF 联合3% DMSO 可诱导BMSCs 向神经元样细胞分化，分化后神经细胞标志物NSE 蛋白表达呈阳性，且神经分化过程中miRNA-26a PTEN/mTOR 的含量明显上调。结论：3%DMSO 和 100 ng·mL-1 BDNF 的混合诱导剂可诱导大鼠BMSCs 向神经元样细胞分化，而miRNA-26a 可能通过PTEN/mTOR 调节神经分化。

关键词: 脑源性神经营养因子, 二甲基亚砜, 骨髓间充质干细胞, 神经分化, miRNA-26a

Abstract:

Objective：To investigate the effect of BDNF combined with DMSO in inducing BMSCs differentiation into neuron-like cells，and to detect the expression of miRNA-26a in the process of neuron differentiation. Methods：BMSCs were isolated from rat femoral bone marrow and preinduced by DMSO. BMSCs were then cultured in a neuronal medium containing BDNF and differentiated into neurons. Differentiated neuron-like cell-specific marker neuron-specific enolase（NSE）was identified using immunohistochemical. Real-time quantitative PCR was used to detect the expression of PTEN/mTOR and miRNA-26a before and after neurodifferentiation. Results：BDNF combined with DMSO had significant effect in inducing BMSCs differentiation into neuron-like cells，and the contents of miRNA-26a and PTEN/mTOR were significantly increased during the process of neurodifferentiation. Conclusion：Mixed inducers of DMSO and BDNF can induce BMSCs differentiation into neuron-like cells in rats，and miRNA-26a may regulate neuronal differentiation through PTEN/mTOR.

Key words: BDNF, DMSO, BMSCs, neurodifferentiation, miRNA-26a

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