神经药理学报

神经药理学报 ›› 2026, Vol. 16 ›› Issue (1): 12-.DOI: 10.3969/j.issn.2095-1396.2026.01.002

• 研究论文 • 上一篇    下一篇

Box-Behnken 响应面法结合三变量单因素筛选泽泻配方颗粒提取工艺研究

李凤丽，孔德伟，张博庸，柴士伟   

  1. 1. 天津中医药大学第一附属医院，天津，300193，中国 

    2. 中医国家临床医学研究中心，天津，300193，中国 

    3. 天津宏仁堂药业有限公司，天津，300385，中国

  • 出版日期:2026-03-02 发布日期:2026-05-07
  • 作者简介:李凤丽，博士，副主任药师；研究方向：中药新药研发
  • 基金资助:
    河北省中医药管理局科研计划项目（No.T2026088）

Optimization of the Extraction Process for Alisma Formula Granules Using the Box-Behnken Response Surface Method Integrated with Three-Variable Single-Factor Screening

LI Feng-li, KONG De-wei, ZHANG Bo-yong, CHAI Shi-wei   

  1. 1. The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China 

    2. National Clinical Research Center for Chinese Medicine, Tianjin, 300193, China 

    3. Tianjin Hongrentang Pharmaceutical Co., Ltd, Tianjin, 300385, China

  • Online:2026-03-02 Published:2026-05-07
  • About author:

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摘要：

目的：筛选泽泻配方颗粒最佳水提取工艺。方法：采用三变量单因素：提取时间、加水量、提取次数为水 平因素，乙酰泽泻C 含量和泽泻出膏率为评价指标，采用 Box-Behnken 响应面法筛选泽泻配方颗粒的水提取工 艺，优选最佳水提取工艺方法。结果：优化后的泽泻配方颗粒水提取工艺为加10 倍量水，煎煮1.5 h，提取3 次。 结论：优选的提取工艺稳定、可行，为泽泻配方颗粒工业化大生产提供依据。

关键词: 泽泻, 配方颗粒, Box-Behnken 响应面法, 23- 乙酰泽泻醇C

Abstract:

Objective: To screen the optimal water extraction process for Alisma orientale formula granules. Methods: A three-variable single-factor method was adopted, with extraction time, water addition volume, and extraction frequency as horizontal factors, and acetyl alisma C content and alisma extract rate as evaluation indicators. The Box-Behnken response surface method was used to screen the water extraction process of Alisma formula granules, and the best water extraction process method was selected. Results: The water extraction process of the optimized Alisma orientale formula granules is to add 10 times the amount of water, boil for 1.5 hours, and extract three times. Conclusion: The preferred extraction process is stable and feasible, providing a basis for the large-scale industrial production of Alisma orientale formula granules.

Key words: alisma orientale, formula granules, Box-Behnken response surface method, 23-acetylalismatol C

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