神经药理学报

神经药理学报 ›› 2012, Vol. 2 ›› Issue (2): 1-6.

• 研究论文 •    下一篇

Protective Effects of Dendrobium Polysaccharides on Learning and Memory Deficits in Aging Mice Induced by D-galactose / 石斛多糖对D-半乳糖诱导衰老小鼠学习记忆损伤的保护作用

王令仪，黄彬，余丽梅，吴芹，张锋，石京山   

  1. 遵义医学院药理学教研室暨贵州省基础药理重点实验室, 遵义, 563099, 中国
  • 出版日期:2012-04-26 发布日期:2013-11-01
  • 通讯作者: 石京山，男，教授，博士生导师；研究方向：神经药理学；Tel:+86-0852-8609788, E-mail: shijs@zmc.edu.cn.
  • 作者简介:王令仪，女，硕士研究生；研究方向：神经药理学；Tel:+86-0852-8609623, E-mail: sunny1984317@163.com
  • 基金资助:

    国家自然科学基金项目(No.30660206、30960447)，贵州省科技厅重点项目（No. 20051001），贵州省中医药管理局(No. C262 )

Protective Effects of Dendrobium Polysaccharides on Learning and Memory Deficits in Aging Mice Induced by D-galactose

WANG Ling-yi, HUANG Bin, YU Li-mei, WU Qin, ZHANG Feng, SHI Jing-shan   

  1. The Key Laboratory of Basic Pharmacology of Guizhou Province and Department of Pharmacology, Zunyi Medical College, Zunyi, 563099, China
  • Online:2012-04-26 Published:2013-11-01
  • Contact: 石京山，男，教授，博士生导师；研究方向：神经药理学；Tel:+86-0852-8609788, E-mail: shijs@zmc.edu.cn.
  • About author:王令仪，女，硕士研究生；研究方向：神经药理学；Tel:+86-0852-8609623, E-mail: sunny1984317@163.com
  • Supported by:

    国家自然科学基金项目(No.30660206、30960447)，贵州省科技厅重点项目（No. 20051001），贵州省中医药管理局(No. C262 )

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摘要： 目的：研究石斛多糖对D-半乳糖诱导衰老小鼠学习记忆损伤的保护作用。方法：通过皮下注射D-半乳糖(120 mg·(kg·d)-1) 60 d诱导小鼠学习记忆损伤模型。观察石斛多糖(80, 160, 320 mg·kg-1)灌胃(ig)60 d后对小鼠学习记忆功能的影响。结果：Morris水迷宫实验、跳台实验（step-down test）和避暗实验（step-through test）显示石斛多糖能够明显改善D-半乳糖诱导的小鼠学习记忆损伤。此外，石斛多糖能够降低脑组织和血浆中丙二醛（malondialdehyde, MDA）的含量以及升高超氧化物歧化酶（superoxide dismutase, SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase, GSH-PX）和Na+-K+-ATP酶的活力。结论：石斛多糖能够保护D-半乳糖诱导的衰老损伤，其作用机制可能与石斛多糖的抗氧化损伤有关。

关键词: 石斛多糖, D-半乳糖, 学习记忆损伤, 抗氧化损伤

Abstract: Objective: To investigate the effects of Dendrobium polysaccharides on D-galactose-induced learning and memory deficits in aging mice. Methods: Adult mice were given subcutaneous injection of D-galactose (120mg·kg-1) daily for 60 d to produce aging-related syndrome, such as learning and memory deficits and alterations in tissue redox status. Dendrobium polysaccharides (80, 160 and 320 mg·kg-1) were administrated by gavage daily for 60 d. Results:Dendrobium polysaccharides significantly ameliorated D-galactose-induced cognitive deficits, as evidenced by the significant performance improvement in the Morris water maze, step-down and step-through tests. Dendrobium polysaccharides also prevented the elevation of malondialdehyde (MDA) in the brain and plasma and restored the antioxidant enzyme activities to normal levels, including superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and Na+-K+-ATPase. Conclusions: Dendrobium polysaccharides is effective against D-galactose-induced aging and this effect appears to be due, at least in part, to its antioxidant activities.

Key words: Dendrobium polysaccharides, D-galactose, cognitive deficits, antioxidant activity

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