神经药理学报

神经药理学报 ›› 2020, Vol. 10 ›› Issue (5): 14-17.DOI: 10.3969/j.issn.2095-1396.2020.05.004

• 实验方法学 • 上一篇    下一篇

气相色谱法测定糖平煎复方中苍术酮含量

田嘉铭，于小红，王银月，王旭，王治宝，王金，卢金铭   

  1. 1. 河北北方学院药学系，张家口，075000，中国
    2. 河北省神经药理学重点实验室，张家口，075000，中国
    3. 河北建筑工程学校，保定，071000，中国
  • 出版日期:2020-10-26 发布日期:2020-10-26
  • 作者简介:田嘉铭，男，硕士，工程师；研究方向：主要从事中药制剂质量控制研究；E-mail：1750345551@qq.com
  • 基金资助:
    张家口市科技局项目（No.1411063C）

Determination of Atractone in Tangpingjian by Gas Chromatography

TIAN Jia-ming，YU Xiao-hong，WANG Yin-yue，WANG Xu，WANG Zhi-bao，WANG Jin，LU Jin-ming   

  1. 1. Department of Pharmacy，Hebei North University，Zhangjiakou，075000，China
    2. Hebei Provincial Key Laboratory of Neuropharmacology，Zhangjiakou，075000，China
    3. Hebei Building Engineering School，Baoding，071000，China
  • Online:2020-10-26 Published:2020-10-26
  • About author:田嘉铭，男，硕士，工程师；研究方向：主要从事中药制剂质量控制研究；E-mail：1750345551@qq.com
  • Supported by:
    张家口市科技局项目（No.1411063C）

PDF (PC)

43

摘要：

目的：建立气相色谱法测定糖平煎中苍术酮含量的方法。方法：色谱柱为PEG-20M 毛细管色谱柱（30 m×0.25 mm×0.25 μm），采用程序升温，进样口温度为290 ℃，检测器温度为300 ℃，检测器为氢火焰离子化检测器（flame ionization detector，FID），载气为氮气，流速1 mL·min-1，内标物质为萘。结果：被测物苍术酮与内标物质萘分离度均良好，平均回收率为100.1%，RSD 为2.64%，糖平煎中苍术酮含量为1.13 mg·g-1。结论：该研究建立的气相色谱方法简便、灵敏度高、数据准确，可用于糖平煎复方中苍术酮含量的测定。

关键词: 糖平煎, 苍术酮, 含量测定, 气相色谱法

Abstract:

Objective：To establish a gas chromatography method for the determination of atractylone in TangpingJian. Method：PEG-20M capillary column （30 m×0.25 mm×0.25 μm） was used as the chromatographic column. The temperature was programmed with the inlet temperature of 290 ℃ and detector temperature of 300 ℃，and the detector was hydrogen flame ionization detector （FID）. The carrier gas was nitrogen with the flow rate of 1 mL·min-1， and naphthalene was used as the internal standard substance. Results：The separation of the atractylone and the internal standard substance was good，and the average recovery rate and RSD was 100.1% and 2.64%，respectively. The atractylone content in the Tangpingjian was 1.13 mg·g-1. Conclusion：The established gas chromatographic method is simple，highly sensitive and accurate. It can be used for the detection of atractylone content in TangpingJian.

Key words: tangpingjian, atractylone, determination, gas chromatography