摘要： Objective：The present study was designed to investigate the effect of resveratrol（Res） on the motor function of Parkinson’s model rats induced by 6-hydroxydopamine （6-OHDA） and to explore its underlying mechanism. Methods：After a week of adaptive feeding，50 male Sprague-Dawley （SD） rats were randomly divided into fi ve groups：sham-operated group，normal group （Res，30 mg·kg-1），model group （6-OHDA，8 μg），6-OHDA+Res low-dose group （15 mg·kg-1，6-OHDA+Res high-dose group （30 mg·kg-1）. 6-OHDA 8 μg （2 μg·μL-1） were injected into the substantia nigra of the rats to establish the model of dopaminergic neuronal damage，while the rats of sham group were injected with volume-matched saline with 0.2% Vitamin C.Rats were pretreated with Res for 1 day before 6-OHDA treatment. Model and sham groups were administered with volume-matched vehicle for 36 days.Rotarod test was applied to evaluate motor function of rats on 7 th，14 th and 21 th day after surgery，open fi eld experiment and grid-walking test applied on 33 th and 35 th day，respectively. Then the rats were sacrificed.Immunohistochemistry was used to detect the number of TH-positive cells in substantia nigra pars compacta （SNc）；the protein expression of TH，Bax，Bcl-2，pro-caspase-3， active-caspase-3，p-PDK1 and p-Akt in midbrain were detected by Western blot. Results：The body weight，motor function，number of dopaminergic neurons in SNc and the protein expression of TH in midbrain of model group were significantly decreased compared with sham group；the protein of Akt phosphorylation levels were decreased while it showed no effect on PDK1，and the expression of apoptosis-related proteins Bax/Bcl-2，active-caspase-3 were significantly increased，pro-caspase-3 decreased. However，compared with model，the body weight，motor function，number of dopaminergic neurons in SNc and the protein expression of TH in midbrain of Res high-dose group were markedly increased；the protein of Akt phosphorylation levels were increased while it showed no effect on PDK1，the protein level of BDNF and TrkB were signifi cantly decreased and the expression of apoptosis-related proteins Bax/Bcl-2，active-caspase-3 were significantly decreased，and pro-caspase-3 were increased.Conclusion：Under the experimental conditions，6-OHDA-induced motor dysfunction and apoptosis in dopaminergic neuronal cells of rats is signifi cantly attenuated by Res，and its potential mechanism may be related to up-regulation of Akt phosphorylation at Ser473 and inhibit the expression of apoptosisrelated proteins.

关键词: Parkinson&rsquo, s disease, Akt, 6-hydroxydopamine, resveratrol, apoptosis