神经药理学报

神经药理学报 ›› 2018, Vol. 8 ›› Issue (2): 30-30.

• 2018年全国老年痴呆与认知障碍相关疾病学术会议论文摘要—基础研究 • 上一篇    下一篇

KAE Alleviates LPS-induced Neuroinflammation in the Striatum of Mice via Down-regulating TLR4/ MyD88 Signaling Pathway

YANG Ying-Lin，CHENG Xiao，LI Wei-Han，LIU Man，WANG Yue-Hua，DU Guan-Hua   

  1. Beijing Key Laboratory of Drug Target Identifi cation and Drug Screening，Institute of Materia Medica，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing，100050，China
  • 出版日期:2018-04-26 发布日期:2018-04-16
  • 通讯作者: 王月华，walling@imm.ac.cn；杜冠华，dugh@imm.ac.cn
  • 基金资助:

    国家自然科学基金面上项目（No.81473383），国家科技重大专项重大新药创制（No.2018ZX09711001- 003-019），中国医学科学院医学与健康科技创新工程（No.2016-I2M-3-007），北京协和医学院研究生创新基金（No.2017-1007-02）

  • Online:2018-04-26 Published:2018-04-16

摘要： Objective：Kaempferol（ KAE） is a natural fl avonoid with many biological activities including anti-oxidation and anti-inflammation. Nevertheless，the relevant mechanism of its antineuroinfl ammation remains unclear. The present study was to investigate the mechanism of KAE against LPS-induced neuroinfl ammation in the striatum of mice. Methods：Lipopolysaccharide （LPS），a lipid polysaccharide，can be used to establish animal inflammation model. Adult male BALB/c mice were treated with KAE 20 mg·kg-1 and 50 mg·kg-1 for 7 days. On the 7th day，the mice were treated with LPS 5 mg·kg-1 for 6 h. The levels of IL-1β，IL-6，TNF-α，MCP-1 and ICAM-1 in the striatum were determined by ELISA. The protein expression of TLR4，MyD88，HMGB1 and P2X7 in the striatum were detected by Western blotting. Results：KAE significantly decreased cytokines release including IL-1β，IL-6，TNF-α，MCP-1 and ICAM-1 in the striatum of mice induced by LPS. In addition，KAE also inhibited the infl ammatory proteins expression，such as TLR4，MyD88，HMGB1 and P2X7，in the striatum of mice induced by LPS. Conclusion：KAE alleviates LPS-induced neuroinfl ammation in the striatum of mice via inhibiting TLR4/MyD88 signaling pathway.

关键词: neuroinfl ammation, striatum, TLR4, MyD88