中药丹参及复方丹参片17种有效成分抗氧化作用的均匀设计-高通量筛选研究

神经药理学报 ›› 2012, Vol. 2 ›› Issue (6): 1-17.

• 研究论文 • 下一篇

中药丹参及复方丹参片17种有效成分抗氧化作用的均匀设计-高通量筛选研究

张丹参

河北科技大学,石家庄,050018,中国

出版日期:2012-12-26 发布日期:2014-06-27
作者简介:张丹参,女,教授,博士,博士生导师；研究方向:中药有效成分抗衰老作用。Tel/Fax:86-311-81668016,E-mail:zhangds2011@126.com
基金资助:
国家自然科学基金项目（No. 81274005、No.81073120）,河北省科技攻关计划项目（No.C2011405014）

The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets

ZHANG Dan-Shen

Hebei University of Science and Technology, Shijiazhuang, 050018, China

Online:2012-12-26 Published:2014-06-27
About author:张丹参,女,教授,博士,博士生导师；研究方向:中药有效成分抗衰老作用。Tel/Fax:86-311-81668016,E-mail:zhangds2011@126.com
Supported by:
国家自然科学基金项目（No. 81274005、No.81073120）,河北省科技攻关计划项目（No.C2011405014）

摘要/Abstract

摘要： 目的:在前期研究的基础上,以丹参及复方制剂复方丹参片有效成分组合活性评价为切入点,通过已经建立的多种抗氧化微孔板试验方法,探讨多因素、多水平均匀设计-高通量筛选中药研究新的模式。方法:选择具有药理活性的丹参有效成分11种（其中脂溶性成分4种,水溶性成分7种）及复方丹参片有效成分6种,通过均匀设计,进行各种成分及不同浓度之间相互配伍组合,以抗2,2-二苯基-1-苦肼基自由基（2,2-diphenyl-1-picrylhydrazyl,DPPH）氧化活性、邻二氮菲-Fe2+氧化法、还原能力测定及抗脂质过氧化法,评价各种有效成分组合对药效的影响。结果:经过初筛和复筛,初步得到了丹参最佳水溶性配伍、水溶性+脂溶性配伍及优化组合配伍的组合样品。结论:丹参各有效成分不同浓度均匀设计配伍,结合抗氧化高通量筛选技术可适用于传统多因素多水平组合特点的大规模药效筛选研究新方法。

关键词: 均匀设计-高通量筛选（UD-HTS）, 丹参, 水溶性成分, 脂溶性成分, 配比

Abstract: Objective: On the basis of the previous studies, and further evaluate and research the bioactive components group of Radix Salviae Miltiorrhiae (RXM) and compound Danshen tablets by multifactor and multilevel Uniform design-High throughput screening method,to find a new pattern to research traditional Chinese medicine. Methods:By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector,11 of bioactive components which been confirmed with pharmacological activities of RXM and compound Danshen tablets(4 of water-solubility and 7 of liposolubility) and Compound Danshen Tablets (6 were recombined to be a new compatible group through uniform design. And the effects of the anti-oxidant microplate quantification experiment modles were detected by anti-DPPH effect,eliminating ratio of hydroxy free radical,reduction ability determination,and lipid peroxidation were established. Results:After high-throughout screening and rescreening, better water-solubility, better water-solubility add liposolubility,and optimal combination compatibility of RXM were obtained. Conclusion:The results indicated that effective component of RXM compatibility of different concentrations of uniform design,and with Uniform design-high throughput screening of anti-oxidant models of microplate quantification was referred to research bioactive component of compound recipe of traditional Chinese medicine.

Key words: Uniform design-High throughput screening(UD-HTS), Radix Salviae Miltiorrhiae(Danshen,RXM), Water soluble constituents, Fat-soluble components, proportion

张丹参. 中药丹参及复方丹参片17种有效成分抗氧化作用的均匀设计-高通量筛选研究[J]. 神经药理学报, 2012, 2(6): 1-17.

ZHANG Dan-Shen. The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets[J]. Acta Neuropharmacologica, 2012, 2(6): 1-17.

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中药丹参及复方丹参片17种有效成分抗氧化作用的均匀设计-高通量筛选研究

The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Component Radix Salviae Miltiorrhiae and Compound Danshen Tablets

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