神经药理学报 ›› 2017, Vol. 7 ›› Issue (2): 49-49.

• 阿尔茨海默病相关研究 • 上一篇    下一篇

The Effect of Dendrobium Nobile Lindl. Alkaloids on the Protein Expression of Aβ42,APP and BACE1 of Hippocampus in db/db Mice

HUANG Juan,ZHANG Ming-hui,XU Yun-yan,YANG Xiao-hui,LIU Yuan,WU Qin,SHI Jing-shan   

  1. Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi,563003,China
  • 出版日期:2017-04-26 发布日期:2017-12-01
  • 通讯作者: SHI Jing-shan,E-mail:zmcshijs@163.com
  • 作者简介:HUANG Juan,E-mail:524586830@qq.com
  • 基金资助:

    The work described in this paper was supported by the Major Project “125” of Guizhou Education Department (Contract serial number 2012-012) project funding and the Major Project of Guizhou Science and Technology Department (Contract serial number 2015-6010).

  • Online:2017-04-26 Published:2017-12-01

摘要: Objective:Beta-amyloid (Aβ) deposition is considered as vital factor leading to cognitive impairment in Alzheimer’s disease( AD).In addition,there are pathophysiological connections between Type 2 Diabetes Mellitus (T2DM) and AD. Diabetic patients have higher incidences of cognitive impairment and hence they are more at the risk of developing AD. As one of the active compounds of Dendrobium nobile Lindl. Dendrobium nobile Lindl. Alkaloids (DNLA) has the effect of protecting the nervous system and decreased the level of fasting blood glucose (FBG) in T2DM model mice. In this study,we attempted to investigate effects of DNLA on the proteins expression of APP,BACE1 and Aβ42 of hippocampus in db/db mice. Methods:10 male C57BL/KsJ mice were
control group. And 4-week-old mice male db/db mice were randomly divided into four groups:model,DNLA-L( 20 mg·kg-1),DNLA-M( 40 mg·kg-1),and DNLA-H (80 mg·kg-1),there are 9 mice in each group. After mice were treated with different concentration DNLA by gavaged for 17 weeks. The protein expression of β-amyloid 42 (Aβ42),β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) and amyloid precursor protein (APP) were examined by Western Blotting. Results:Compared with control group,not only the protein expression of Aβ42,but also BACE1 and APP was signifi cantly increased in hippocampus of model group. Moreover,DNLA signifi cantly decreased the protein expressions of Aβ42,BACE1 and APP in hippocampus of db/db mice compared with model group,and decreased in a dosedependent manner. Conclusion:DNLA can decrease the protein expressions of Aβ42 in hippocampus of db/ db mice. And the mechanism may be involved the decrease of BACE1 and APP.

关键词: Alzheimer&rsquo, s disease, Dendrobium nobile Lindl. Alkaloids, db/db mice, APP, BACE1, A&beta, 42