黄芪甲苷对PC12细胞缺糖缺氧损伤的保护作用

神经药理学报 ›› 2015, Vol. 5 ›› Issue (1): 10-14.

黄芪甲苷对PC12细胞缺糖缺氧损伤的保护作用

杨文亮，宋玉，赵灿灿，谢欣梅，庞晓斌，杜冠华

1. 河南大学药学院药物研究所，开封，475004，中国
2. 中国医学科学院&北京协和医学院药物研究所国家药物筛选中心，北京，100050，中国

出版日期:2015-02-26 发布日期:2015-07-06
通讯作者: 庞晓斌，男，副教授，硕士生导师；研究方向：神经药理学
作者简介:杨文亮，男，硕士研究生；研究方向：神经药理学；E-mail: 434500839@qq.com
基金资助:
国家自然科学基金资助项目 (No. 81273652)

Protective Effect of Astragaloside Ⅳ on PC12 Cells Injured by Oxygen-glucose Deprivation

YANG Wen-liang, SONG Yu, ZHAO Can-can, XIE Xin-mei, PANG Xiao-bin, DU Guan-hua

1. Pharmaceutical Institute, Pharmaceutical College of Henan University, Kaifeng 475004, China
2. National Centre for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China

Online:2015-02-26 Published:2015-07-06
Contact: 庞晓斌，男，副教授，硕士生导师；研究方向：神经药理学
About author:杨文亮，男，硕士研究生；研究方向：神经药理学；E-mail: 434500839@qq.com
Supported by:
国家自然科学基金资助项目 (No. 81273652)

摘要/Abstract

摘要： 目的：探究黄芪甲苷对PC12细胞缺糖缺氧损伤的保护作用，并探讨其可能的作用机制。方法：体外培养PC12细胞，应用无糖Earle’s稀释Na2S2O4建立缺糖缺氧模型，尼莫地平做阳性对照，使用不同浓度的黄芪甲苷进行预保护。显微镜下观察细胞形态差异；以MTT法测定细胞活力；酶标法测定上清液中LDH含量；Western-blot法测定Bcl-2与Bax蛋白表达量。结果：黄芪甲苷能够提高PC12细胞在缺糖缺氧环境下的细胞存活率，降低LDH漏出量；能够增加Bcl-2蛋白的表达，降低Bax蛋白的表达，降低Bax/Bcl-2比值。结论：黄芪甲苷对PC12细胞的缺糖缺氧损伤具有一定的保护作用，其作用机制可能与其抑制细胞凋亡有关。

关键词: 黄芪甲苷, PC12细胞, 缺糖缺氧损伤, 细胞凋亡

Abstract: Objective：To investigate the protective effect and mechanism of astragaloside Ⅳ on PC12 cells injured by oxygen-glucose deprivation. Methods：The oxygen-glucose deprivation model of PC12 cells was constructed with Na2S2O4 diluted with glucose-free Earle’s. PC12 cells were treated with different doses of astragaloside Ⅳ. Nimodipine was taken as a positive control. The cellular morphology difference was observed and the cell survival rate was detected with MTT method. The lactate dehydrogenase（ LDH） concentration was determined with enzyme linked immunosorbent assay. The expression of Bcl-2 and Bax protein were detected by Western blot. Results：The oxygen glucose deprivation（ OGD） model was successfully established. In contrast with the OGD group，astragaloside Ⅳ could increase the survival rate of PC12 cells and reduce LDH leakage. Astragaloside Ⅳ could also increase the expression of Bcl-2 protein and decrease the expression of Bax protein. Conclusion：Astragaloside Ⅳ could relieve the PC12 cell injury induced by OGD and the protective effect may be related to inhibiting cell apoptosis.

Key words: Astragaloside Ⅳ, PC12 cell, Oxygen glucose deprivation, Apoptosis

中图分类号:

R965

杨文亮, 宋玉, 赵灿灿, 谢欣梅, 庞晓斌, 杜冠华. 黄芪甲苷对PC12细胞缺糖缺氧损伤的保护作用[J]. 神经药理学报, 2015, 5(1): 10-14.

YANG Wen-liang, SONG Yu, ZHAO Can-can, XIE Xin-mei, PANG Xiao-bin, DU Guan-hua. Protective Effect of Astragaloside Ⅳ on PC12 Cells Injured by Oxygen-glucose Deprivation[J]. ACTA NEUROPHARMACOLOGICA, 2015, 5(1): 10-14.

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