瞬时受体电位M7通道与血小板源性生长因子和5-HT促进大鼠肺动脉平滑肌细胞增殖相关性的研究

神经药理学报 ›› 2012, Vol. 2 ›› Issue (3): 15-21.

瞬时受体电位M7通道与血小板源性生长因子和5-HT促进大鼠肺动脉平滑肌细胞增殖相关性的研究

杲海霞¹，乔晓温² ，敦洁宁³，董明纲⁴，徐志伟⁴ ，张海林¹

1 河北医科大学药理学教研室，石家庄，050000，中国
2辛集市中医院儿科，辛集，052360，中国

3河北医科大学药剂教研室，石家庄，050000，中国
4河北北方学院，张家口，075000，中国

出版日期:2012-06-26 发布日期:2013-12-25
通讯作者: 张海林，河北医科大学药理学教研室；E-mail：z.hailin@yahoo.com
作者简介:杲海霞，河北医科大学药理学教研室；Tel+86-0311-86261032，E-mail：gaohx686@yahoo.com
基金资助:
国家自然科学基金青年基金（No.81201642）

Transient Receptor Potential Melastatin 7 Involved in the Proliferation Induced by Platelet Derived Growth Factor and 5-HT in Pulmonary Artery Smooth Muscle Cells of Rates

GAO Hai-xia¹, QIAO Xiao-wen², DUN Jie-ning³, DONG Ming-gang⁴，XU Zhi-wei⁴, ZHANG Hai-lin¹

1 The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Department of Pharmacology, Hebei Medical University, Shijiazhuang, 050000, China
2 Department of Pediatrics, Xinji Hospital of Traditional Chinese Medicin, 052360, China

3 Department of Pharmacy, Hebei Medical University, Shijiazhuang, 050000, China
4 Hebei North University, Zhangjiakou, 075000, China

Online:2012-06-26 Published:2013-12-25
Contact: 张海林，河北医科大学药理学教研室；E-mail：z.hailin@yahoo.com
About author:杲海霞，河北医科大学药理学教研室；Tel+86-0311-86261032，E-mail：gaohx686@yahoo.com
Supported by:
国家自然科学基金青年基金（No.81201642）

摘要/Abstract

摘要： 目的：探讨瞬时受体电位M7通道（transient receptor potential melastatin 7, TRPM7）在血小板源性生长因子（platelet derived growth factor, PDGF）和5-羟色胺（5-hydroxytryptamine, 5-HT）诱导的肺动脉平滑肌细胞（pulmonary artery smooth muscle cells, PASMCs）异常增殖中的作用。方法：利用贴块法和酶解法分离大鼠PASMCs。采用RT-PCR、Western blot和全细胞膜片钳技术观察TRPM7在PASMCs中的功能性表达。利用TRPM7阻断剂2-APB，采用MTT的方法检测TRPM7对PDGF和5-HT诱导的PASMCs异常增殖的作用。结果：RT-PCR、Western blot结果显示，TRPM7在大鼠PASMCs中有mRNA和蛋白表达。利用膜片钳技术检测到TRPM7电流，且PDGF可上调TRPM7内、外向电流。100 μmol•L^-1 2-APB可抑制PDGF和5-HT引起的大鼠PASMCs异常增殖。结论：TRPM7可能参与PDGF和5-HT诱导的大鼠PASMCs的增殖，有可能成为肺动脉高压临床治疗新的靶点。

关键词: 瞬时受体电位M7, 血小板源性生长因子, 5-羟色胺, 肺动脉平滑肌细胞, 肺动脉高压

Abstract: Objective: To investigate the role of transient receptor potential melastatin7 (TRPM7) in platelet derived growth factor (PDGF) and 5-hydroxytryptamine (5-HT)-induced rat pulmonary artery smooth muscle cells (PASMCs) abnormal proliferation. Methods: PASMCAs were cultured with the explant technique. The expression of TRPM7 was evaluated by RT-PCR, western blot and whole-cell patch clamp. The proliferation of PASMCs was evaluated by MTT assay. TRPM7 inhibitor 2-APB was used to investigate the receptor mechanism of TRPM7-like current and the effects of TRPM7 in abnormal proliferation induced by PDGF and 5-HT. Results: RT-PCR and western blot showed that the TRPM7 mRNA and protein were detected in rat PASMCs. Electrophysiological results showed that the TRPM7 currents were detectable and that PDGF can increase the amplitude of TRPM7 inside and outside currents. 100 μmol•L^-1 2-APB inhibited PDGF and 5-HT-induced abnormal proliferation in rat PASMCs. Conclusion: TRPM7 participates in PDGF and 5-HT-induced abnormal proliferation in rat PASMCs and TRPM7 may be a new target for clinical treatment of pulmonary artery hypertension (PHA).

Key words: transient receptor potential melastatin7 (TRPM7), platelet derived growth factor (PDGF), 5-hydroxytryptamine (5-HT), platelet derived growth factor (PASMCs), pulmonary artery hypertension (PAH)

中图分类号:

R349.5

杲海霞，乔晓温，敦洁宁，董明纲，徐志伟，张海林. 瞬时受体电位M7通道与血小板源性生长因子和5-HT促进大鼠肺动脉平滑肌细胞增殖相关性的研究[J]. 神经药理学报, 2012, 2(3): 15-21.

GAO Hai-xia, QIAO Xiao-wen, DUN Jie-ning, DONG Ming-gang，XU Zhi-wei,ZHANG Hai-lin. Transient Receptor Potential Melastatin 7 Involved in the Proliferation Induced by Platelet Derived Growth Factor and 5-HT in Pulmonary Artery Smooth Muscle Cells of Rates[J]. Acta Neuropharmacologica, 2012, 2(3): 15-21.

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