神经药理学报 ›› 2020, Vol. 10 ›› Issue (2): 19-23.DOI: 10.3969/j.issn.2095-1396.2020.02.005

• 实验方法学 • 上一篇    下一篇

冬凌草黄酮分散片含量测定方法研究

李港,张季,王新利,王亚男,石宗杨   

  1. 1 河北大学药学院,保定,071000,中国
    2 河北北方学院药学系,张家口,075000,中国
  • 出版日期:2020-04-26 发布日期:2020-04-26
  • 通讯作者: 张季,女,硕士,副教授;研究方向:中药药理学与药剂学研究;E-mail:zhangjizhji@126.com
  • 作者简介:李港,男,硕士在读;研究方向:药物化学;E-mail:1210920834@qq.com

Study on the Content Determination Method of Flavone Dispersible Tablets of Herba Rabdosiae

LI Gang,ZHANG Ji,WANG Xin-Li,WANG Ya-nan,SHI Zong-yang   

  1. 1 College of Pharmacy,Hebei University,Baoding,071000,China
    2 Department of Pharmacy,Hebei North University,Zhangjiakou,075000,China
  • Online:2020-04-26 Published:2020-04-26
  • Contact: 张季,女,硕士,副教授;研究方向:中药药理学与药剂学研究;E-mail:zhangjizhji@126.com
  • About author:李港,男,硕士在读;研究方向:药物化学;E-mail:1210920834@qq.com

摘要:

目的:建立紫外分光光度法测定冬凌草黄酮分散片中黄酮含量的方法。方法:用紫外分光光度计,在410 nm 波长处,通过三氯化铝显色法,建立芦丁标准品的标准曲线,用标准曲线法测定冬凌草黄酮分散片中黄酮的含量,并且用小杯法在恒温37℃下测定冬凌草黄酮分散片的溶出度。结果:显示芦丁在浓度为0.001~0.080 mg·mL-1范围内有良好的线性关系,线性回归方程Y=28.388X+0.017 6,R2=0.999 4。冬凌草黄酮分散片最终累计溶出量为86.20%,几乎全部溶出,溶出速率快。结论:用三氯化铝显色法通过紫外分光光度法测定冬凌草黄酮分散片中黄酮含量具有操作便捷,准确性很好,灵敏度很高,稳定性好的优点,并且冬凌草黄酮分散片的溶出度很好说明此分散片是一个快速释放的制剂,而且本方法操作简便、准确、快速,且符合方法学要求。

关键词: 冬凌草, 分散片, 黄酮, 紫外可见分光光度法, 含量

Abstract:

Objective:In order to determine the content of flavone in Herba Rabdosiae flavone dispersible tables simply and accurately. Methods:Building ultraviolet spectrophotometry method to the content of flavone in Herba Rabdosiae flavone dispersible tablets. Through chloride color development method and ultraviolet spectrophotometer with a detection wavelength of 410 nm to build standard substance of rutin of standard curve. Detect the content of flavone in Herba Rabdosiae flavone dsipersible tablets through the standard curve and the dissolution rate of Herba Rabdosiae flavone dsipersible tablets with little cup at 37℃. Results:The results showed that the linear relationship was good in the concentration range of 0.001~0.080 mg·mL-1,and the linear regression equation was Y=28.388X+0.017 6,R2=0.999 4. The dissolution rate of Herba Rabdosiae flavone dsipersible tablets dissolved 86.20% at the end. Almost all of them dissolved,and the dissolution rate was fast. Conclusion:Instruct the method of chloride color development to content of flavone in Herba Rabdosiae flavone dsipersible tablets is simply and accurately. The method have good veracity,high sensitivity and gong stability. Herba Rabdosiae flavone dsipersible tablets have good dissolution rate shows that they are good dsipersible tablets. And this method have many advantages like simple,accurate,quick and so on.

Key words: Herba Rabdosiae, dispersible tablets, flavone, UV, assaying