Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF

doi:10.3969/j.issn.2095-1396.2019.05.002

Acta Neuropharmacologica

Previous Articles Next Articles

Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF

NI Shuang，MIAO Ze-yuan，WANG Jia-xin，et al

1. Anesthesia Major, Basic Medical College of Hebei North University，Zhangjiakou，075000, China
2. Clinical Speciality, Basic Medical College of Hebei North University, Zhangjiakou，075000, China
3. Stomatology, Basic Medical College of Hebei North University, Zhangjiakou，075000, China
4. Department of Biology, Basic Medical College of Hebei North University, Zhangjiakou, 075000, China
5. Editorial Department of Journal of Hebei North University，Zhangjiakou，075000，China

Online:2019-10-26 Published:2020-03-03
Contact: 苏立宁，讲师；研究方向：细胞生物学；E-mail：343376274@qq.com
About author:倪爽，本科在校生；研究方向：医学麻醉
Supported by:
2018 年国家级大学生创新创业训练计划项目（No.201810092001），河北北方学院青年基金项目（No.QN2018022）

Abstract

Abstract:

Objective：To investigate the effect of BDNF combined with DMSO in inducing BMSCs differentiation into neuron-like cells，and to detect the expression of miRNA-26a in the process of neuron differentiation. Methods：BMSCs were isolated from rat femoral bone marrow and preinduced by DMSO. BMSCs were then cultured in a neuronal medium containing BDNF and differentiated into neurons. Differentiated neuron-like cell-specific marker neuron-specific enolase（NSE）was identified using immunohistochemical. Real-time quantitative PCR was used to detect the expression of PTEN/mTOR and miRNA-26a before and after neurodifferentiation. Results：BDNF combined with DMSO had significant effect in inducing BMSCs differentiation into neuron-like cells，and the contents of miRNA-26a and PTEN/mTOR were significantly increased during the process of neurodifferentiation. Conclusion：Mixed inducers of DMSO and BDNF can induce BMSCs differentiation into neuron-like cells in rats，and miRNA-26a may regulate neuronal differentiation through PTEN/mTOR.

Key words: BDNF, DMSO, BMSCs, neurodifferentiation, miRNA-26a

CLC Number:

R965

NI Shuang，MIAO Ze-yuan，WANG Jia-xin，et al. Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF[J]. Acta Neuropharmacologica, DOI: 10.3969/j.issn.2095-1396.2019.05.002.

References

[1] Feng Yan, Ju Yaru, Cui Jian-zhong, et al. Bone marrow stromal cells promote neuromotor functional recovery, via upregulation of neurotrophic factors and synapse proteins following traumatic brain injury in rats [J]. Mol Med Rep, 2017, 16(1):654-660.
[2] Lim Jung-Yeon, Sang In Park, Ji Hyeon Oh, et al. Brain-derived neurotrophic factor stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival of differentiated cells through MAPK/ERK and P13 K/Akt-dependent signaling pathways [J]. J Neurosci Res, 2008, 86(10): 2168-2178.
[3] Dale Woodbury, Kathleen Reynolds, Ira B Black. Adult bone marrow stromal stem cell express germline , ectodermal , endodermal , and mesodermal genesprior to neurogenesis[J]. Neurosci Res, 2002, 69(6): 908 - 917.
[4] Andrew S Yoo, Brett T Staahl, Chen Lei, et al. Microrna-mediated switching of chromatin-remodelling complexes in neural development[J]. Nature, 2009, 460(7255): 642-646.
[5] Sarah K Fineberg, Kenneth S Kosik, Beverly L Davidson. Micrornas potentiate neural development [J]. Neuron, 2009, 64(3): 303-309.
[6] Lena Smirnova, Anja Grafe, Andrea Seiler, et al. Regulation of mirna expression during neural cell specification [J]. Eur J Neurosci, 2005, 21(6): 1469-1477.
[7] Lv Xiao-hui, Jiang Hui-hui, Liu Yan-li, et al. MicroRNA-15b promotes neurogenesis and inhibits neural progenitor proliferation by directly repressing TET3 during early neocortical development[J]. EMBO Rep, 2014, 15(12):1305-1314.
[8] Shin J, Yoojin Shin, Oh S M, et al. MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling[J]. Cell Death Dis, 2014, 5: e1473.
[9] 李保国. miR-26a 对小鼠神经元形态发育影响的研究[D]. 广州：暨南大学, 2013.
[10] J Sanchez Ramos, Song S, F Cardozo-Pelaez, et al. Adult bone marrow stromal cells differentiate into neural cells in vitro[J]. Exp Neurol, 2000, 164(2): 247-256.
[11] Ma Jun, Zhang Zhan-chi, Su Yu-hong, et al. Magnetic stimulation modulates structural synaptic plasticity and regulates BDNF-TrkB signal pathway in cultured hippocampal neurons [J]. Neurochem Int, 2013, 62(1): 84-91.
[12] Al-Ali H, Ding Ying, Tatiana I Slepak, et al. The mTOR substrate S6 Kinase 1 (S6K1) is a negative regulator of axon regeneration and a potential drug target for Central Nervous System injury[J]. J Neurosci, 2017, 37(30): 931-917.
[13] Su Li-ning, Song Xiao-qing, Xue Zhan-xia, et al. Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration[J]. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 2018, 19(4):293-304.
[14] 卢璐，周恒星，褚天慈，等. 慢病毒介导的shrna干扰pten表达促皮层神经元轴突再生及脊髓损伤修复[J]. 中华骨科杂志, 2015, 35(4): 428-435.
[15] Kevin Kyungsuk Park, Liu Kai, Hu Yang, et al. Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway[J]. Science, 2008, 322(5903): 963-966.
[16] Huang Zheng-ru, Hu Zi-zhong, Xie Ping, et al. Tyrosine-mutated AAV2-mediated shRNA silencing of PTEN promotes axon regeneration of adult optic nerve[J]. PLoS One, 2017, 12(3): e0174096.

Differentiation of BMSCs into Neuron-Like Cells Induced by DMSO Combined with Neurotrophic Factor BDNF

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Recommended Articles

Metrics

[1]	ZHANG Qun-feng，HE Huan-ying，LIAO Yun-hao，WANG Guo-qing. The Effects of Maternal Chlorpyrifos Exposure on the Expression of COX2，CREB and BDNF in Hippocampus of Offspring Mice [J]. Acta Neuropharmacologica, 2019, 9(6): 1-5.
[2]	WANG Wei-sheng，JU Yun-yue，WANG Yu-jun，LIU Jing-gen. The Small GTPase Rac1 Contributes to Extinction of Aversive Memories of Drug Withdrawal by Facilitating GABAA Receptor Endocytosis in the vmPFC [J]. Acta Neuropharmacologica, 2018, 8(5): 65-66.
[3]	WEI Hui-ping, ZHU Deng- xiang, ZHANG Ai-lan, SONG Xiao-qing, CUI Le. *Repairment of In Vitro* Induced Neuron-like Cells on the Brain Injury in Rats** [J]. Acta Neuropharmacologica, 2013, 3(6): 20-24.
[4]	YAO Guang-Yin, JI Dan-Yang, ZHOU Wei-Dong, YAO Hai-Yan, KU Bao-Shan, YANG Dong-Hui. Secoisolariciresinol Reduces Behavioral Despair and Regulates Hippocampal BDNF Expression in Ovariectomized Mice [J]. Acta Neuropharmacologica, 2013, 3(2): 13-25.
[5]	YUAN Yu-he，WU Miao-miao，HU Jin-feng，CHEN Nai-hong. α-Synuclein Inhibits the Expression of BDNF in PC12 Cells [J]. Acta Neuropharmacologica, 2011, 1(4): 1-5.