Acta Neuropharmacologica

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The Effect of Scutellaria Barbata Flavonoid Against N2a Cells Injury Induced by Aβ25-35

CAI Hong-ling,CHENG Jian-jun,PAN Qi,MIAO Hong,SHANG Ya-zhen   

  1. Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia,Hebei Provincial Key Laboratory of Traditional Chinese Medicine Research and Development,Institute of Traditional Chinese Medicine,Chengde Medical College,Chengde,067000,China
  • Online:2017-10-26 Published:2017-12-01
  • Contact: 商亚珍,女,博士,硕士生导师,教授;研究方向:中药抗痴呆;Tel:+86-0313-2290616,E-mail:shangyz1018@sina.com
  • About author:蔡红玲,女,硕士研究生;研究方向:神经药理学;Tel:+86-0313-2290616,E-mail:1225820994@qq.com
  • Supported by:
    河北省自然基金资助项目(No.C2009001007、No.H2014406048),河北省中医药管理局资助项目(No.05027、No.2014062)

Abstract:

Objective:To investigate the effect of scutellaria barbata fl avonoid against N2a cells injury induced by β-amyloid protein 25-35 (Aβ25-35). Methods:N2a cells were cultured in vitro and randomly divided into control group,model group,scutellaria barbata flavonoid (SBF)1.125 μg·mL-1,2.250 μg·mL-1 and 4.500 μg·mL-1 group. After the N2a cells of three doses of group were treated by 1.125 μg·mL-1,2.250 μg·mL-1 and 4.500 μg·mL-1 SBF for 12 h,the
model and SBF group cells were exposed to Aβ25-35 at the end concentration of 35 μmol·L-1. The morphology of N2a cells was observed and photographed under an inverted microscope. The level of lactate dehydrogenase (LDH) in the culture medium was measured by spectrophotography. The
cell viability was determined by thiazolyl blue tetrazolium bromide (MTT). Results:Compared with control group,the N2a cells were found markedly injured in morphology and structure, including the damage of the link between cells and cells,some sells agglomeration and refractive index decrease,the level of LDH in the culture medium of N2a cells significantly increased (P<0.01) and the N2a cell viability obviously reduced (P<0.01) in model group. Compared with the model group,three dose of SBF could dramatically oppose the cells injured in morphology, including the cells neurite growth,cell structure clearness and refractive index increase,lower the level of LDH of the cells’ culture medium (P<0.05) and add the cell viability (P<0.01).
Conclusion:SBF can significantly oppose the injury of N2a cells induced by Aβ25-35.

Key words: scutellaria barbata flavonoid, β-amyloid protein 25-35(Aβ25-35), N2a cells, lactate dehydrogenase, cell viability

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