关键词: 半枝莲黄酮, β- 淀粉样蛋白25-35, N2a 细胞, 乳酸脱氢酶, 细胞存活率

Abstract:

Objective：To investigate the effect of scutellaria barbata fl avonoid against N2a cells injury induced by β-amyloid protein 25-35 （Aβ25-35）. Methods：N2a cells were cultured in vitro and randomly divided into control group，model group，scutellaria barbata flavonoid （SBF）1.125 μg·mL-1，2.250 μg·mL-1 and 4.500 μg·mL-1 group. After the N2a cells of three doses of group were treated by 1.125 μg·mL-1，2.250 μg·mL-1 and 4.500 μg·mL-1 SBF for 12 h，the
model and SBF group cells were exposed to Aβ25-35 at the end concentration of 35 μmol·L-1. The morphology of N2a cells was observed and photographed under an inverted microscope. The level of lactate dehydrogenase （LDH） in the culture medium was measured by spectrophotography. The
cell viability was determined by thiazolyl blue tetrazolium bromide （MTT）. Results：Compared with control group，the N2a cells were found markedly injured in morphology and structure， including the damage of the link between cells and cells，some sells agglomeration and refractive index decrease，the level of LDH in the culture medium of N2a cells significantly increased （P<0.01） and the N2a cell viability obviously reduced （P<0.01） in model group. Compared with the model group，three dose of SBF could dramatically oppose the cells injured in morphology， including the cells neurite growth，cell structure clearness and refractive index increase，lower the level of LDH of the cells’ culture medium （P<0.05） and add the cell viability （P<0.01）.
Conclusion：SBF can significantly oppose the injury of N2a cells induced by Aβ25-35.

Key words: scutellaria barbata flavonoid, β-amyloid protein 25-35（Aβ25-35）, N2a cells, lactate dehydrogenase, cell viability