神经药理学报

神经药理学报 ›› 2015, Vol. 5 ›› Issue (6): 6-10.

• 研究论文 • 上一篇    下一篇

NGF、NCAM、CXCL12/CXCR4在涎腺腺样囊性癌嗜神经侵袭中作用的研究

杜贵霞1, 张 凡2, 刘 博2, 李瑞平3, 刘军超2, 韩向春2, 张九鸿2, 李 坤2, 赵秀芳2   

  1. 1．河北北方学院，张家口，075000，中国
    2 .河北北方学院附属第一医院病理科，张家口，075000，中国
    3．河北北方学院附属第一医院口腔科，张家口，075000，中国
  • 出版日期:2015-12-26 发布日期:2016-03-03
  • 通讯作者: 张凡，男，硕士研究生导师，教授；研究方向：肿瘤分子病理
  • 作者简介:杜贵霞，女，山东临沂人，硕士研究生
  • 基金资助:

    河北省卫生厅计划项目（No.06108）；河北北方学院计划项目（No.2007036）；张家口市科技局计划项目（No.0711045D-7）

The Study on the Role of NGF、NCAM、CXCL12/CXCR4 in Salivary Adenoid Cystic Carcinoma Perineural Invasion

DU Gui-xia1, ZHANG Fan2, LIU Bo2, LI Rui-ping3, LIU Jun-chao2, HAN Xiang-chun2, ZHANG Jiu-hong2, LI Kong2, ZHAO Xiu-fang2   

  1. 1. Hebei North University, Zhangjiakou, 075000， China 
    2. Department of Pathology, The First Affiliated Hospital, Hebei North University. Zhangjiakou, 075000，China
    3. Department of Stomatology, The First Affiliated Hospital, Hebei North University. Zhangjiakou, 075000，China
  • Online:2015-12-26 Published:2016-03-03
  • Contact: 张凡，男，硕士研究生导师，教授；研究方向：肿瘤分子病理
  • About author:杜贵霞，女，山东临沂人，硕士研究生
  • Supported by:

    河北省卫生厅计划项目（No.06108）；河北北方学院计划项目（No.2007036）；张家口市科技局计划项目（No.0711045D-7）

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摘要： 目的：探讨涎腺腺样囊性癌（salivary adenoid cystic carcinoma，SACC）中神经生长因子（nerve growth factor，NGF ）、神经细胞黏附分子（neural cell adhesion molecule，NCAM）、趋化因子（CXC chemokine ligand 12，CXCL12 ）及趋化因子受体（CXC chemokine receptor type 4，CXCR4）表达与嗜神经侵袭的关系。方法：选取临床病理资料齐全的SACC 60例，分为嗜神经侵袭组（36例）和非嗜神经侵袭组（24例），制作组织芯片。采用免疫组织化学（immunohistochemistry，IHC）技术检测两组NGF、NCAM、CXCL12/CXCR4蛋白的表达，分析指标表达与临床病理参数的相关性。结果：NGF、CXCL12、CXCR4蛋白在SACC嗜神经侵袭组阳性表达率均高于非嗜神经侵袭组，差异具有统计学意义（P＜0.05）；NGF、CXCL12、CXCR4表达与患者性别、组织学类型、肿瘤发生部位及大小均无关（P＞0.05）。SACC中NGF与CXCL12的阳性表达相关（r=0.279，P＜0.05），也与CXCR4阳性表达相关（r=0.451，P＜0.05），但CXCL12与CXCR4无明显相关（r=0.254，P=0.05）。NCAM在两组SACC中均呈阴性表达。结论：NGF、CXCL12/CXCR4过表达共同参与了SACC的嗜神经侵袭过程。

关键词: 涎腺腺样囊性癌, 嗜神经侵袭, 组织芯片, 神经生长因子, 神经细胞黏附分子, 趋化因子

Abstract: Objective：To investigate the relationship among nerve growth factor (NGF), neural cell adhesion molecule (NCAM), CXC chemokine ligand 12(CXCL12), CXC chemokine receptor type 4 (CXCR4) and perineural invasion in salivary adenoid cystic carcinoma (SACC). Methods：We Choose 60 cases of SACC with complete clinical data, divided them into perineural invasion group and non perineural invasion group, marking every cases according to slide and made tissue assay. Using immunohistochemistry technique to detect the expression of NGF、NCAM、CXCL12/CXCR4 in the two group, we analysis the relationship  between factor and the clinical pathological parameters. Results：The ratio of NGF、CXCL12、CXCR4 protein’s positive expression in SACC perineural invasion group were more higher than that of the non perineural invasion group, there are magnificent difference between them (P＜0.05); there are no relationship between the expression of NGF、CXCL12、CXCR4 and patient's sex, histological types, tumor location and size(P＞0.05). There are positive relationship between the expression of NGF and CXCL12 (r=0.279, P＜0.05)and CXCR4 (r=0.451, P＜0.05), but there are no positive relationship between expression of CXCL12 and CXCR4 (P＞0.05). There are no expressions of NCAM in the two groups. Conclusion：NGF、CXCL12/CXCR4 all take part in the perineural invasion of SACC.

Key words: salivary adenoid cystic carcinoma (SACC), perineural invasion, tissue chip, NGF , NCAM , CXCL12

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