生物样品中胍丁胺的定量分析方法及评价

神经药理学报 ›› 2015, Vol. 5 ›› Issue (2): 9-16.

生物样品中胍丁胺的定量分析方法及评价

张玉瑶，姜国华，班涛

1．黑龙江中医药大学基础医学院解剖与组胚学教研室，哈尔滨，150040，中国
2．哈尔滨医科大学药学院药理学教研室，哈尔滨，150086，中国

出版日期:2015-04-26 发布日期:2015-07-06
通讯作者: 班涛，男，副教授，硕士研究生导师；研究方向：神经药理学研究；Tel：+86-0451-86671354，E-mail：bantao2000@163.com
作者简介:张玉瑶，女，讲师，硕士；研究方向：神经科学研究；Tel：+86-0451-82193433，E-mail：zyyhhcc@163.com
基金资助:
黑龙江省青年科学基金项目（No.QC2012C061），黑龙江省教育厅基金科学技术研究项目面上项目（No. 12531252），心血管药物研究教育部重点实验室（哈尔滨医科大学）开放课题项目（No. 2014014）

Summary of Quantitative Analysis and Correlation Evaluation of Agmatine Content in Biological Samples

ZHANG Yu-yao, JIANG Guo-hua, BAN Tao

1. Department of Anatomy, Histology and Embryology, Heilongjiang University of Chinese Medicine, Harbin, 150040, China
2. Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin, 150086, China

Online:2015-04-26 Published:2015-07-06
Contact: 班涛，男，副教授，硕士研究生导师；研究方向：神经药理学研究；Tel：+86-0451-86671354，E-mail：bantao2000@163.com
About author:张玉瑶，女，讲师，硕士；研究方向：神经科学研究；Tel：+86-0451-82193433，E-mail：zyyhhcc@163.com
Supported by:
黑龙江省青年科学基金项目（No.QC2012C061），黑龙江省教育厅基金科学技术研究项目面上项目（No. 12531252），心血管药物研究教育部重点实验室（哈尔滨医科大学）开放课题项目（No. 2014014）

摘要/Abstract

摘要： 胍丁胺是精氨酸脱羧基生成的内源性代谢产物，在自然界中广泛存在于植物、细菌及哺乳类动物的多种组织中。研究表明胍丁胺有着重要的生物学特性如增强吗啡镇痛，降低吗啡耐受及躯体依赖等作用，故具有良好的新药开发前景。准确而快速地测定生物体内的胍丁胺对于研究其生物学功能具有十分重要的意义。目前，已有多种测量方法用于胍丁胺的定量测定，如色谱法、质谱法、电泳法、免疫法等。该文对胍丁胺含量测定方法的研究进展、适用范围及其优缺点作一综述，旨在后期对胍丁胺的深入研究提供方法学的参考。

关键词: 胍丁胺, 高效液相色谱法, 气相色谱/质谱联用, 毛细管电泳法, 酶联免疫吸附实验

Abstract: Agmatine which presents in plants, bacteria and several mammalian tissues is an endogenous amine synthesized from the decarboxylation of arginine. Agmatine has many physiological functions, such as enhancing morphine analgesia, reducing the symptoms of dependence and tolerance, and so on. It has a good prospect of application in new drug development. For a better understanding of its physiological functions, the exact and fast determination of agmatine in biological samples is necessary. A variety of measurement methods including chromatography, mass spectrometry, electrophoresis and immunoassays had been used for the determination of agmatine levels. In this article, the research progresses on determination of agmatine in biological samples are reviewed; the advantages and disadvantages of the different determination methods and their applications are also discussed, hence to provide methodology reference for the analysis of agmatine.

Key words: agmatine, high performance liquid chromatography (HPLC), gas chromatography-mass spectrum (GC/MS), capillary electrophoresis (CE), Enzyme linked immuno-sorbent assay (Elisa)

张玉瑶，姜国华，班涛. 生物样品中胍丁胺的定量分析方法及评价[J]. 神经药理学报, 2015, 5(2): 9-16.

ZHANG Yu-yao, JIANG Guo-hua, BAN Tao. Summary of Quantitative Analysis and Correlation Evaluation of Agmatine Content in Biological Samples[J]. Acta Neuropharmacologica, 2015, 5(2): 9-16.

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