神经药理学报

神经药理学报 ›› 2012, Vol. 2 ›› Issue (6): 36-39.

• 实验方法学 • 上一篇    下一篇

正交试验优化南五味子多糖的超声提取工艺

谭晓虹，王治宝，卫娇娇，张亚飞，卢金铭   

  1. 1 河北北方学院药学系，张家口，075000，中国
    2 华北电力大学科技学院，保定，071000，中国
  • 出版日期:2012-12-26 发布日期:2014-06-27
  • 作者简介:谭晓虹， 女，硕士，副教授，主要从事中药有效成分分析与药代动力学研究。E-mail ：TXHZBWZL@ 126. com
  • 基金资助:

    河北省中医药管理局资助项目（No. 2013076）

Study on Extraction Polysaccharide from Fructus Schisandrae Sphenantherae by Orthogonal Ultrasound-Assisted Method

TAN Xiao-hong，WANG Zhi-bao，WEI Jiao-jiao，ZHANG Ya-fei，LU Jin-ming   

  1. 1 Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China
    2 North China Electric Power University Science and Technology College, Baoding, 071000, China
  • Online:2012-12-26 Published:2014-06-27
  • About author:谭晓虹， 女，硕士，副教授，主要从事中药有效成分分析与药代动力学研究。E-mail ：TXHZBWZL@ 126. com
  • Supported by:

    河北省中医药管理局资助项目（No. 2013076）

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摘要： 目的：优化南五味子多糖的提取条件。方法：采用正交试验设计，以南五味子多糖提取率为考察指标，考察提取时间（extraction time）、固液比（solid-liquid ratio）和浸泡时间（immersion time）对多糖提取率的影响。结果：试验表明南五味子多糖提取所需的最优时间为40min，浸泡最佳时间为90min，固液比为1:25，在此条件下南五味子多糖提取率较高。结论：优选出的最佳提取条件，试验证实结果稳定，方法可行，为南五味子多糖的进一步研究提供参考。

关键词: 南五味子, 多糖, 超声辅助, 正交试验

Abstract: Objective: To optimize the extraction condition for polysaccharide of Fructus Schisandrae Sphenantherae. Methods: The extraction technique was designed by orthogonal test with extraction time，solid-liquid ratio and immersion time as factors using extraction rate as index. Results：The optimum conditions for the ultrasound-assisted method to extract polysaccharide were as follows: extraction time was 40min, solid-liquid ratio was 1:25, immersion time was 90min. Conclusion：The optimum process is feasible, stable and reasonable. It provide reference for the further study of the polysaccharide of Fructus Schisandrae  

Key words: fructus schisandrae sphenantherae, polysaccharide, ultrasound-assisted, orthogonal test