小鼠条件性电击社交恐惧行为模型的建立与评价

神经药理学报 ›› 2014, Vol. 4 ›› Issue (4): 15-19.

小鼠条件性电击社交恐惧行为模型的建立与评价

赵楠, 张黎明, 李云峰

军事医学科学院毒物药物研究所，北京，100850，中国

出版日期:2014-08-26 发布日期:2015-01-20
通讯作者: 李云峰，男，博士，研究员，研究方向：精神药理学，E-mail：lyf619@aliyun.com
作者简介:赵楠，女，山东省蓬莱市人，药理学硕士，实验师，E-mail: zhaonan203040@163.com

Establishment and Evaluation of the Conditioned Foot-shock as a Behaviour Model of Social Phobia in Mice

ZHAO Nan, ZHANG Li-Ming, LI Yun-Feng

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, 100850, China

Online:2014-08-26 Published:2015-01-20
Contact: 李云峰，男，博士，研究员，研究方向：精神药理学，E-mail：lyf619@aliyun.com
About author:赵楠，女，山东省蓬莱市人，药理学硕士，实验师，E-mail: zhaonan203040@163.com

摘要/Abstract

摘要： 目的：建立稳定而特异性强的小鼠社交恐惧行为模型。方法：采用针对社交接触行为给予条件性足底电击（0.7 mA, 1s, 1~5次）的方式建立小鼠社交恐惧模型。分别于电击后d 2和d 15检测小鼠的社交探究行为，以探究时间百分比（investigation time %, IT %）为社交恐惧行为评价指标。此外，分别采用小鼠高架十字迷宫模型、明暗穿箱模型和强迫性游泳模型，以评价小鼠的广泛性焦虑行为和抑郁样行为。结果：在条件性电击后d 2和d 15的社交探究实验中，与非社交刺激（non-social stimulus, NSS）组比较，条件性社交刺激（conditioned social stimulus, CSS）组小鼠 IT % 均显著减少。在小鼠高架十字迷宫模型、明暗穿箱模型和强迫性游泳模型上，CSS组与NSS组各行为指标均无显著差异。结论：采用条件性足底电击方式可成功建立小鼠社交恐惧行为模型，模型症状可持续至少2 w，且不伴随广泛性焦虑行为或抑郁样行为，具有良好的稳定性和特异性。

关键词: 社交恐惧, 行为模型, 社交探究行为, 小鼠

Abstract: Objective：To establish a stable and specific behaviour model of social phobia(SP) in mice. Methods: The conditioned foot-shock (0.7mA, 1s, 1-5 times) was used to induce a model of SP in mice. The social investigation behavior were detected on d 2 or d 15 after the foot-shock, with the percentage of investigation time ( IT % ) as a behavior index of SP. In addition, the elevated plus maze test (EPMT), the light/dark transition test (L/DTT) and the forced swimming test (FST) were used to evaluate the generalized anxiety behavior or depression-like behavior of the model mice. Results: In the social investigation test, compared with the non-social stimulus（NSS）group, the IT % of conditioned social stimulus（CSS） group were significantly decreased on d 2 or d 15 day after the conditioned foot-shock. On the other hand, no significant differences were observed between NSS group and CSS group on the behavioral indexes of EPMT, L/DTT and FST. Conclusion: The SP model in mice was established successfully by the conditioned foot-shock paradigm. The model was stable and specific within at least 2 weeks and without the interference of generalized anxiety or depression-like behavior.

Key words:

social phobia, animal model, social investigation behavior, ICR mice

中图分类号:

R965

赵楠, 张黎明, 李云峰. 小鼠条件性电击社交恐惧行为模型的建立与评价[J]. 神经药理学报, 2014, 4(4): 15-19.

ZHAO Nan, ZHANG Li-Ming, LI Yun-Feng. Establishment and Evaluation of the Conditioned Foot-shock as a Behaviour Model of Social Phobia in Mice[J]. Acta Neuropharmacologica, 2014, 4(4): 15-19.

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