神经药理学报

神经药理学报 ›› 2012, Vol. 2 ›› Issue (4): 8-12.

• 研究论文 • 上一篇    下一篇

金莲花中荭草苷和牡荆苷对D-半乳糖致衰老小鼠脑组织动态抗氧化作用研究

田青青,安芳,王书华   

  1. 河北北方学院，张家口，075000，中国
  • 出版日期:2012-08-26 发布日期:2014-06-27
  • 通讯作者: 王书华，教授，硕士生导师；研究方向：中药有效成分提取及其药理活性研究；E-mail：wangshyh@126.com
  • 作者简介:田青青，研究生；研究方向：药物分析；E-mail:tianqingqing900927@126.com
  • 基金资助:

    河北省张家口市科技局资助项目（No.11110015D），河北北方学院（No.ZD1314）

Study on Dynamical-antioxidation of Orientin and Vitexin in Trollius Chinensis to Brain Tissue in D-Galactose-induced aging Mice

TIAN Qing-qing, AN Fang ,WANG Shu-hua   

  1. College of Pharmacy, Hebei North University, Zhangjiakou, 075000, China
  • Online:2012-08-26 Published:2014-06-27
  • Contact: 王书华，教授，硕士生导师；研究方向：中药有效成分提取及其药理活性研究；E-mail：wangshyh@126.com
  • About author:田青青，研究生；研究方向：药物分析；E-mail:tianqingqing900927@126.com
  • Supported by:

    河北省张家口市科技局资助项目（No.11110015D），河北北方学院（No.ZD1314）

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摘要： 目的：研究金莲花中荭草苷和牡荆苷对D-半乳糖(D-galactose，D-gal）致衰老小鼠脑组织抗氧化酶活性的动态影响，寻找荭草苷和牡荆苷对脑组织抗氧化酶系活性作用的时效关系。方法：采用D-gal腹腔注射（intraperitoneal injection, ip）致亚急性衰老小鼠为模型，8周造模成功后，分成正常组，模型组，荭草苷和牡荆苷各三个剂量组（40，20，10 mg·kg-1），维生素E对照组。各治疗组从第9周开始每日上午分别口服灌胃(intragastric perfusion, ig)给药，每日一次。给药第2，4，6，8周时，分别断头处死小鼠，用预冷的生理盐水制成10％的脑组织匀浆，8000 r离心得上清液，检测超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶以及脑脂褐素含量。结果：与模型组相比，给药2、4周后，各治疗组抗氧化酶系虽有提高但无统计学意义，给药6、8周后，各治疗组在提高抗氧化酶系活性以及降低脑脂褐素含量上差异有显著性。荭草苷和牡荆苷相比，给药2、4周后，各剂量组在提高抗氧化酶活性及降低脑脂褐素含量上无统计学意义，给药6周后，荭草苷40 mg·kg-1，20 mg·kg-1，10 mg·kg-1三个剂量组在提高抗氧化酶活性及降低脑脂褐素含量上优于同等剂量的牡荆苷，给药8周后，荭草苷20 mg·kg-1和10 mg·kg-1剂量组在提高抗氧化酶系的活性及降低脑脂褐素含量上高于同等剂量的牡荆苷，而40 mg·kg-1剂量组差异无显著性。与维生素E相比，给药8周后，荭草苷和牡荆苷20 mg·kg-1，10 mg·kg-1两个剂量组在提高脑组织抗氧化酶系活性及降低脑脂褐素含量上低于维生素E，40 mg·kg-1剂量组差异无显著性。结论：D-gal致衰老小鼠脑组织抗氧化酶活性的作用，随给药时间和给药剂量的增加，其提高抗氧酶活性的作用增强。高剂量的荭草苷和牡荆苷抗氧作用与维生素E相当。荭草苷和牡荆苷具有提高

Abstract: Objective: To study antioxidant enzymes activity and lipofuscin dynamic effects of orientin and vitexin in Trollius chinensis on aging mice induced by D-Galactose(D-gal) and find the time effect relationship of antioxidant. Methods: The aging mice model were induced by intraperitoneal injection of D-gal, and it was successful after 8 weeks. Divided into blank group, modelgroup, high, medium and low dose group of orientin and vitexin, Vitamin E control group. From the 9 th week, every morning each group will be given medicine. The route is oral administration, and the time is 8 weeks. Decapitated mice at the 2 th , 4 th , 6 th and 8 th week , resected brain in frozen conditions, 10% tissue homogenate were made with NS solution, then got supernatant by use of centrifugation in 8000 r, detected superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GSH-PX) and lipofuscin(LPF). Results: Compared with model group, the activity of antioxidase in each therapy group increased slowly after 2 and 4 weeks, but not statistically significant , and after 6 and 8 weeks, orientin and vitexin significantly improved the activity of antioxidase, and significantly reduced the amount of LPF. Compared orientin with vitexin, the activity of antioxidase and the amount of LPF in the same dose group was no statistically significant after 2 and 4 weeks. The activity of antioxidase in each dose group of orientin were better than that of the vitexin of the same dose. they significantly reduced lipofuscin levels in the brain after 6 weeks and orientin was superior to vitexin at the same dose. The activity of antioxidase in medium and low dose group of orientin was better than the same vitexin, they significantly reduced lipofuscin levels in the brain and high dose group have no significant difference after 8 weeks．Compared with VE group, after 8 weeks, the activity of antioxidase in medium and low dose group of orientin and vitexin were lower than VE group, they have significant difference, meanwhile, high dose group have no significant difference. Conclusion：Orientin and Vitexin can apparently improve activity of antioxidation and reduce lipofuscin levels in brain. The activity of antioxidase became increased with the concentration increasing and reaction time prolonging. After 8 weeks, high dose group of orientin and vitexin have the same function with VE.

Key words: orientin, vitexin, D-galactose, antioxidation, brain tissue

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