低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响

神经药理学报 ›› 2015, Vol. 5 ›› Issue (4): 8-13.

低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响

李双，王小琴，郭春燕，邹玉安

1 河北北方学院，张家口，075000，中国
2 河北北方学院第一附属医院，张家口，075000，中国

出版日期:2015-08-26 发布日期:2016-03-03
通讯作者: 郭春燕，女，教授，博士，研究生导师；研究方向：中药药效组分，中药组分对神经损伤保护作用；Email：guochy0311@163.com
作者简介:李双，2013级药学本科
基金资助:
河北省教育厅重大项目（项目编号:ZD2014075）

Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells

LI Shuang，WANG Xiao-qin，GUO Chun-yan，ZOU Yu-an

1. Hebei North University, Zhangjiakou, 075000, China
2. The First Affiliated Hospital of Hebei North, Zhangjiakou, 075000, China

Online:2015-08-26 Published:2016-03-03
Contact: 郭春燕，女，教授，博士，研究生导师；研究方向：中药药效组分，中药组分对神经损伤保护作用；Email：guochy0311@163.com
About author:李双，2013级药学本科
Supported by:
河北省教育厅重大项目（项目编号:ZD2014075）

摘要/Abstract

摘要： 目的：研究二甲基亚砜（dimethyl sulfoxide，DMSO）对SH-SY5Y细胞存活率和氨基酸代谢的影响。方法：将细胞分为正常组和DMSO组。0.0125%浓度的DMSO作用SH-SY5Y细胞不同时间（24，48 h）后，利用MTT法测定细胞存活率；高效液相色谱法测定细胞中氨基酸的含量。结果：与正常组相比，在0.0125% DMSO作用下，细胞活力和形态无显著差异，但细胞内氨基酸含量变化有显著差异（P<0.05）。其中作用24 h，细胞中有7种氨基酸：谷氨酸（glutamic acid，Glu）、谷氨酰胺（glutamine，Gln）、甘氨酸（glycine，Gly）、脯氨酸（proline，Pro）、苯丙氨酸（phenylalanine，Phe）、异亮氨酸（isoleucine，Ile）、亮氨酸（leucine，Leu）含量有显著变化（P<0.05）；作用48 h，细胞中有12种氨基酸：谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、苯丙氨酸、异亮氨酸、亮氨酸、丝氨酸（serine，Ser）、组氨酸（histidine，His）、赖氨酸（lysine，Lys）、丙氨酸（alanine，Ala）、色氨酸（tryptophan，Trp）含量有显著变化（P<0.01）。结论：细胞活力无显著差异时，细胞氨基酸代谢已有明显变化，氨基酸代谢异常可能为细胞损伤早期的一种特征。

关键词: 氨基酸, 高效液相色谱, SH-SY5Y细胞, 二甲基亚砜

Abstract: Objective: The main objective of this article is to estimating the effect of dimethyl sulfoxide (DMSO) on livability and amino acid metabolism of SH-SY5Y cells. Methods: SH-SY5Y cells were divided into normal group and DMSO group. The DMSO group was exposed to 0.0125% DMSO in different time (24, 48 h) to investigate amino acid transform which was associated with cell damage.SH-SY5Y cell livability was determined by MTT as well as amino acids content was determined by high performance liquid chromatography. Results: Compared with the normal group, DMSO group`s 7 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine) decreased significantly after SH-SY5Y cells were treated with DMSO for 24 h (P<0.05),and 12 amino acids (glutamic acid, glutamine, glycine, proline, phenylalanine, isoleucine, leucine, serine, histidine, lysine, alanine, tryptophan) decreased significantly after treated with DMSO for 48 h (P<0.01). But there was no significant transform in cell vitality and morphological specificity. Conclusions: Although there was no obvious change in cell vitality and morphological specificity, but amino acid concentrations had significant transform. This finding suggests that abnormal amino acid metabolism may be a potential early and causal feature of cell damage.

Key words: amino acid, high performance liquid chromatography, SH-SY5Y cells, dimethyl sulfoxide

李双, 王小琴, 郭春燕, 邹玉安. 低浓度DMSO 对SH-SY5Y 细胞存活率和氨基酸代谢的影响[J]. 神经药理学报, 2015, 5(4): 8-13.

LI Shuang, WANG Xiao-qin, GUO Chun-yan, ZOU Yu-an. Effect of Low Level of DMSO on Cell Survival Rate and Amino Acid Metabolism in SH-SY5Y Cells[J]. ACTA NEUROPHARMACOLOGICA, 2015, 5(4): 8-13.

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