蛇床子素脂质体的制备及其质量评价

神经药理学报 ›› 2015, Vol. 5 ›› Issue (4): 26-32.

蛇床子素脂质体的制备及其质量评价

张成龙，张智峰，孟宪勇，董晓华

1. 河北北方学院，张家口，075000，中国
2. 中国人民解放军93508部队医院，北京，100000，中国
3. 河北北方学院附属第一医院，张家口，075000，中国

出版日期:2015-08-26 发布日期:2016-03-03
通讯作者: 董晓华，女，副教授，硕士生导师；研究方向：神经药理学；E-mail: hbdxh76@163.com
作者简介:张成龙，男，硕士研究生；研究方向：神经药理学；E-mail:386017477@qq.com
基金资助:
河北省科技支撑项目（No.13272704D），河北省教育厅重点项目（No.ZD2014065），张家口市科技局资助项目（No.0921098D），河北北方学院创新人才项目（No.CXRC1320）

Preparation and Quality Evaluation of Osthole Liposome

ZHANG Cheng-long, ZHANG Zhi-feng, MENG Xian-yong, DONG Xiao-hua

1. Hebei North University, Zhangjiakou, 075000, China
2. 93508 Military Hospital of Chinese People's Liberation Army, Beijing, 100000, China
3. The First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, China

Online:2015-08-26 Published:2016-03-03
Contact: 董晓华，女，副教授，硕士生导师；研究方向：神经药理学；E-mail: hbdxh76@163.com
About author:张成龙，男，硕士研究生；研究方向：神经药理学；E-mail:386017477@qq.com
Supported by:
河北省科技支撑项目（No.13272704D），河北省教育厅重点项目（No.ZD2014065），张家口市科技局资助项目（No.0921098D），河北北方学院创新人才项目（No.CXRC1320）

摘要/Abstract

摘要： 目的: 对蛇床子素脂质体最佳制备工艺及处方进行研究，并评价其质量。方法: 采用薄膜-超声分散法制备蛇床子素脂质体，以包封率为评价指标，采用单因素和正交实验优化蛇床子素脂质体的制备工艺和处方，并测定其粒径和Zeta电位。结果: 蛇床子素脂质体优化后的制备工艺和处方为：胆固醇与大豆卵磷脂质量比为1:3，药脂比为3:20，pH值为7.9，成膜温度为45 ℃。按此处方工艺制备蛇床子素脂质体包封率在80%以上。平均粒径为（466.6 ± 6.4）nm，平均Zeta电位为（-29.0 ± 1.8）mV。结论: 优选的处方和工艺条件可行且稳定，制备的蛇床子素脂质体包封率高，稳定性好。

关键词: 蛇床子素, 脂质体, 薄膜-超声分散法, 正交试验

Abstract: Objective：To research the best preparation technology and prescription, and to evaluate its quality. Methods：Osthole liposome was prepared through film-ultrasonic dispersion method. Encapsulation efficiency was taken as index, the preparation technology and prescription were optimized through single factor and orthogonal test, and particle size and Zeta potential were measured. Results: The optimal formulations were cholesterol/lecithin=1:3, drug/lecithin =3:20, pH=7.9, and at 45℃ reactive temperature. The entrapment efficiency was over 80%. Average particle size was（466.6 ± 6.4）nm and Zeta potential was（-29.0 ± 1.8）mV. Conclusion：The preparation prescription and technology were feasible and stable, and osthole liposome has high entrapment efficiency and good stability.

Key words: osthole, liposome, film-ultrasonic dispersion method, orthogonal test

张成龙，张智峰，孟宪勇，董晓华. 蛇床子素脂质体的制备及其质量评价[J]. 神经药理学报, 2015, 5(4): 26-32.

ZHANG Cheng-long, ZHANG Zhi-feng, MENG Xian-yong, DONG Xiao-hua. Preparation and Quality Evaluation of Osthole Liposome[J]. Acta Neuropharmacologica, 2015, 5(4): 26-32.

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